Home LiteratureArticle Details
PMID: 6057809 Published · ppublish English Journal Article

Multiple alterations in metabolite uptake in a mutant of Neurospora crassa.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1629-37

Kappy MS, Metzenberg RL

Abstract

A temperature-conditional lethal mutant of Neurospora crassa, un-t (55701), was resistant to neutral amino acid analogues by virtue of a decreased ability to transport these analogues and their natural congeners across the cell membrane. The uptake of acidic, but not basic, amino acids was also impaired, as was the uptake of potassium ions. After preincubation above the tolerated temperature, the ability to take up a still wider variety of metabolites was greatly reduced. Protoplasts of the mutant were more osmotically fragile than those of wild type. The possibility that the mutant has a generalized membrane defect is discussed.

MeSH Terms
Acetates/metabolism Amino Acids/metabolism Biological Transport Carbon Isotopes Cell Membrane Cell Membrane Permeability Cycloheximide/pharmacology Ethionine/metabolism Fluorine Glucose/metabolism Mutation Neurospora/growth & development,metabolism Osmotic Fragility Phenylalanine/metabolism Phosphates/metabolism Potassium/metabolism Potassium Isotopes Protein Biosynthesis Protoplasts Sulfates/metabolism Sulfur Isotopes Temperature
Chemicals
Acetates Amino Acids Carbon Isotopes Phosphates Potassium Isotopes Sulfates Sulfur Isotopes Fluorine Phenylalanine Cycloheximide Glucose Potassium Ethionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kappy M S
Metzenberg R L
References (35)
35 references, click to expand
  1. Genetic control of the uptake of amino acids in Neurospora.
    Genetics. 1966 Aug;54(2):677-85 PMID: 5968647
  2. Protoplasts from Neurospora crassa.
    J Bacteriol. 1959 Oct;78:550-6 PMID: 13795550
  3. The invertase isozyme formed by Neurospora protoplasts.
    Biochem Biophys Res Commun. 1964 Jul 1;16(4):319-25 PMID: 5871816
  4. CARBOHYDRATE TRANSPORT IN STAPHYLOCOCCUS AUREUS I. GENETIC AND BIOCHEMICAL ANALYSIS OF A PLEIOTROPIC TRANSPORT MUTANT.
    Biochim Biophys Acta. 1965 Feb 15;97:310-9 PMID: 14292840
  5. Regulation of sulfate transport in Salmonella typhimurium.
    J Bacteriol. 1966 Jun;91(6):2275-80 PMID: 5329286
  6. Recombination and interference in the ad-3 region of Neurospora crassa.
    Cold Spring Harb Symp Quant Biol. 1958;23:111-8 PMID: 13635548
  7. INTERALLELIC RECOMBINATION AT THE HIS-5 LOCUS IN NEUROSPORA CRASSA.
    Heredity (Edinb). 1965 May;20:257-76 PMID: 14313687
  8. BIOCHEMICAL MECHANISMS OF DRUG RESISTANCE.
    Annu Rev Microbiol. 1964;18:347-66 PMID: 14268863
  9. Bacterial permeases.
    Bacteriol Rev. 1957 Sep;21(3):169-94 PMID: 13471457
  10. Tryptophan transport in Neurospora crassa. I. Specificity and kinetics.
    J Bacteriol. 1966 Dec;92(6):1698-705 PMID: 5958105
  11. AMINO ACID ACCUMULATION IN ETHIONINE-RESISTANT SACCHAROMYCES CEREVISIAE.
    J Bacteriol. 1964 Jul;88:20-4 PMID: 14197889
  12. IRREPARABLE MUTATIONS AND ETHIONINE RESISTANCE IN NEUROSPORA.
    Science. 1964 Sep 25;145(3639):1434-5 PMID: 14172634
  13. THE EFFECT OF TEMPERATURE ON THE PRODUCTION OF PERITHECIA BY NEUROSPORA CRASSA.
    J Gen Microbiol. 1965 Apr;39:33-42 PMID: 14328412
  14. UPTAKE OF AMINO ACIDS BY SALMONELLA TYPHIMURIUM.
    Arch Biochem Biophys. 1964 Jan;104:1-18 PMID: 14110716
  15. THE ASSIMILATION OF ARGININE AND LYSINE IN CANAVANINE RESISTANT AND SENSITIVE STRAINS OF NEUROSPORA CRASSA.
    Biochim Biophys Acta. 1964 Nov 8;93:316-22 PMID: 14251310
  16. EFFECTS OF AMINO ACIDS ON THE UTILIZATION OF TRYPTOPHAN AND INDOLE FOR GROWTH BY A MUTANT OF NEUROSPORA CRASSA.
    J Gen Microbiol. 1964 Jan;34:31-41 PMID: 14121218
  17. [Resistance to ethionine in Saccharomyces cerevisiae. II. Physiological study].
    Genetics. 1966 Oct;54(4):993-1006 PMID: 5972438
  18. MUTATIONAL ALTERATION OF PERMEABILITY IN NEUROSPORA: EFFECTS ON GROWTH AND THE UPTAKE OF CERTAIN AMINO ACIDS AND RELATED COMPOUNDS.
    Genetics. 1964 Dec;50:1383-402 PMID: 14239795
  19. POTASSIUM TRANSPORT IN NEUROSPORA. I. INTRACELLULAR SODIUM AND POTASSIUM CONCENTRATIONS, AND CATION REQUIREMENTS FOR GROWTH.
    Biochim Biophys Acta. 1964 Nov 29;88:578-92 PMID: 14249101
  20. Genetic control of amino acid permeability in Neurospora crassa.
    J Bacteriol. 1966 Feb;91(2):677-84 PMID: 5883112
  21. The enzymatic lesion of strain MM-6, a pleiotropic carbohydrate-negative mutant of Escherichia coli.
    Biochem Biophys Res Commun. 1967 Apr 7;27(1):63-7 PMID: 4862174
  22. An impaired concentrating mechanism for amino acids in mutants of Escherichia coli resistant to L-canavanine and D-serine.
    Biochim Biophys Acta. 1959 Apr;32:582-3 PMID: 14443991
  23. Mitotic recombination in pseudo-wild types of Neurospora.
    Genetics. 1965 Sep;52(3):609-25 PMID: 5863909
  24. Carbohydrate transport in Staphylococcus aureus. 3. Studies of the transport process.
    Biochim Biophys Acta. 1966 Jan 4;112(1):63-73 PMID: 5947899
  25. Studies on the basis of ethionine-resistance in Neurospora.
    Biochim Biophys Acta. 1965 Oct 18;107(3):425-33 PMID: 5867300
  26. Effect of the alpha-hydrazino analogue of histidine on histidine transport and arginine biosynthesis.
    J Biol Chem. 1966 Jul 25;241(14):3424-9 PMID: 5330432
  27. Potassium transport in Neurospora. 3. Isolation of a transport mutant.
    Biochim Biophys Acta. 1965 Sep 27;109(1):184-93 PMID: 5864012
  28. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  29. A Map of Linkage Group VI of Neurospora Crassa.
    Genetics. 1956 Jul;41(4):528-43 PMID: 17247646
  30. Altered repression of some enzymes of sulfur utilization in a temperature-conditional lethal mutant of Neurospora.
    Proc Natl Acad Sci U S A. 1966 Mar;55(3):629-35 PMID: 5221246
  31. The isolation of bacterial mutants defective in amino acid transport.
    Biochim Biophys Acta. 1960 Aug 26;42:535-8 PMID: 13763941
  32. Molecular transport in Neurospora crassa. I. Biochemical properties of a phenylalanine permease.
    Biochim Biophys Acta. 1965 Jun 15;104(1):139-50 PMID: 5840393
  33. On the genetics of enzyme locational specificity.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1217-24 PMID: 5225520
  34. Cysteine mutant strains of Neurospora.
    Genetics. 1965 Oct;52(4):801-8 PMID: 5826323
  35. Determining mating type in Neurospora without crossing tests.
    Nature. 1961 Jun 10;190:1036 PMID: 13716257
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.94.5.1629-1637.1967
Published
1967-11-00
Pages
1629-37
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276872
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.94.5.1629-1637.1967 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2