Physiochemical properties of the Vi antigen before and after mild alkaline hydrolysis.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1406-10

Jarvis FG, Mesenko MT, Martin DG, Perrine TD

Abstract

Sedimentation and diffusion constants were determined for electrophoretically purified Vi antigen before and after mild alkaline hydrolysis. The molecular weight of the intact antigen was found to be 1.7 x 10(6). Mild alkaline hydrolysis completely removed O-acetyl, reduced the molecular weight to 1.0 x 10(5), and lowered the intrinsic viscosity from 8.0 deciliters/g to approximately 0.5 deciliter/g. Infrared spectra of the two antigen preparations were also compared.

MeSH Terms

Alkalies Antigens Electrophoresis Escherichia Infrared Rays Molecular Weight Spectrum Analysis Ultracentrifugation Viscosity

Chemicals

Alkalies Antigens

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Jarvis F G

Mesenko M T

Martin D G

Perrine T D

References (11)

11 references, click to expand

Physicochemical and biological properties of sonically treated Vi antigen.
J Bacteriol. 1967 Nov;94(5):1411-6 PMID: 4964477
Studies on Vi antigen. II. Purification of Vi antigen from Escherichia coli 5395/38.
J Immunol. 1952 Aug;69(2):135-42 PMID: 14946409
Evidence for an aminohexuronic acid as hydrolytic product of Vi antigen.
Arch Biochem Biophys. 1954 May;50(1):223-4 PMID: 13159320
Studies on Vi antigen. IV. Physicochemical characterization of Vi antigens isolated from V form Enterobacteriaceae.
J Immunol. 1954 Jul;73(1):16-22 PMID: 13184128
An aminohexuronic acid as the principal hydrolytic component of the Vi antigen.
J Biol Chem. 1958 Jan;230(1):81-9 PMID: 13502376
Studies on Vi antigen. VIII. Role of acetyl in antigenic activity.
Am J Hyg. 1961 Jan;73:55-65 PMID: 13758705
Electrophoretic purification of the Vi antigen.
J Bacteriol. 1960 Nov;80:677-82 PMID: 13789617
Production of Vi antigen on a chemically defined medium by a coliform bacterium.
J Bacteriol. 1960 Nov;80:673-6 PMID: 13789618
Immunological properties of Vi antigen isolated by chemical fractionation and by electrophoresis.
Tex Rep Biol Med. 1963;21:214-29 PMID: 13928496
Studies on the antigenic substances of Eberthella typhosa.
Jpn J Exp Med. 1949 Sep;20(2):181-209 PMID: 15407944
The VI antigens of the Enterobacteriaceae. I. Purification and chemical properties.
J Immunol. 1959 Dec;83:680-96 PMID: 13795984

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.94.5.1406-1410.1967

Published

1967-11-00

Pages

1406-10

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC276839

Subset

External Links

PubMed source DOI: 10.1128/jb.94.5.1406-1410.1967 PMC Full Text View journal details

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Physiochemical properties of the Vi antigen before and after mild alkaline hydrolysis.

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