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PMID: 6033763 Published · ppublish English Journal Article

Enzymes in cancer. Asparaginase from chicken liver.

The Biochemical journal ·Vol. 103 ·No. 1 ·1967-04-00 ·Pages 238-45

Ohnuma T, Bergel F, Bray RC

Abstract

1. A procedure for partial purification of asparaginase from chicken liver is presented. 2. The bulk of the enzyme is located in the soluble fraction of chicken liver. 3. Molecular weights of chicken-liver asparaginase and of the guinea-pig serum enzyme, estimated by gel filtration, were 306000 and 210000 respectively. The Michaelis constants (K(m)) at 37 degrees and pH8.5 were 6.0x10(-5)m and 7.2x10(-5)m respectively. 4. At 50 degrees the chicken-liver enzyme was moderately stable, some activity being lost by aggregation; in dilute electrolyte solutions the activity rapidly diminished. 5. The anti-lymphoma effect of guinea-pig serum in mice carrying the 6C3HED tumour was confirmed. Chicken-liver asparaginase also showed an effect but in this case the enzyme preparation had to be administered repeatedly. 6. Guinea-pig serum asparaginase was stable for several days in mouse blood, after intraperitoneal injection, whereas chicken-liver asparaginase rapidly disappeared. 7. Aspartic acid beta-hydrazide was shown to be a competitive inhibitor of chicken-liver asparaginase with K(i) approx. 5.6x10(-4)m. In mice it produced an anti-lymphoma effect, as reported previously.

MeSH Terms
Amidohydrolases/analysis,therapeutic use Animals Aspartic Acid/pharmacology Cattle Chromatography, Gel Enzymes/blood Guinea Pigs Hydrazines/pharmacology Kinetics Liver/enzymology Lymphoma/drug therapy Mice Molecular Weight Neoplasms, Experimental/drug therapy Poultry Rabbits Rats
Chemicals
Enzymes Hydrazines Aspartic Acid Amidohydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ohnuma T
Bergel F
Bray R C
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030238
Published
1967-04-00
Pages
238-45
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270391
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030238 PMC Full Text View journal details
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