Mechanisms of regulation of urease biosynthesis in Proteus rettgeri.

Urease of Proteus rettgeri is an inducible enzyme synthesized specifically in the presence of urea; urea analogues did not act as inducers. Once initiated, the biosynthesis of the enzyme proceeded as a constant fraction of the total protein formed. The rate of urease formation was affected by the carbon source used. In comparison with glycerol, glucose inhibited enzyme synthesis. The addition of ammonium ions to the inducing medium also decreased the rate of urease biosynthesis, and when ammonium ions were present urease activity and urea transport across the cell membrane were inhibited. A kinetic analysis of urease inhibition by ammonium ions, by use of a partially purified preparation of urease, showed that it was a competitive inhibition.