The separation of the alpha-chains of collagen by free-flow electrophoresis.

The Biochemical journal ·Vol. 102 ·No. 1 ·1967-01-00 ·Pages 148-52

Francois CJ, Glimcher MJ

Abstract

1. The separation of the alpha-components of codfish-skin and calf-skin collagen from the higher-molecular-weight components was accomplished by gel filtration with Bio-Gel P-300 resin in 5m-lithium chloride and at pH7.4. 2. From this fraction three distinct alpha-components, corresponding to the alpha1-, alpha2- and alpha3- chains of collagen, were isolated by free-flow electrophoresis in 6m-urea at 4 degrees . At the temperatures and pH values used there was no detectable degradation of the alpha-chains.

MeSH Terms

Animals Cattle Chromatography Chromatography, Gel Collagen/analysis Electrophoresis Fishes In Vitro Techniques Methylcellulose Peptides/analysis Skin

Chemicals

Peptides Methylcellulose Collagen

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Francois C J

Glimcher M J

References (12)

12 references, click to expand

Characterization of a collagen from codfish skin containing three chromatographically different alpha chains.
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A modified procedure for the automatic analysis of amino acids.
Anal Biochem. 1960 Nov;1:187-201 PMID: 13735644
The long range reorganization of gelatin to the collagen structure.
Arch Biochem Biophys. 1961 Jul;94:20-31 PMID: 13780601
Subunits of the collagen molecule.
Biochim Biophys Acta. 1961 Nov 11;53:596-8 PMID: 14486552
SEDIMENTATION-EQUILIBRIUM STUDIES OF THE MOLECULAR WEIGHT OF SINGLE AND DOUBLE CHAINS FROM RAT-SKIN COLLAGEN.
Biochemistry. 1964 Aug;3:1126-31 PMID: 14220677
DISC ELECTROPHORESIS OF COLLAGEN COMPONENTS.
Ann N Y Acad Sci. 1964 Dec 28;121:494-500 PMID: 14240547
NONIDENTITY OF THE 3 ALPHA CHAINS IN CODFISH SKIN COLLAGEN.
J Biol Chem. 1964 Dec;239:4315-6 PMID: 14247688
THE PRESENCE OF ORGANIC PHOSPHORUS IN COLLAGENS AND GELATINS.
Biochim Biophys Acta. 1964 Dec 9;93:585-602 PMID: 14263157
Gamma tropocollagen: a reversibly denaturable collagen macromolecule.
Proc Natl Acad Sci U S A. 1961 Dec 15;47:1914-24 PMID: 13860730
The separation and characterization of the alpha- and beta-components of calf skin collagen.
J Biol Chem. 1960 Jul;235:1987-91 PMID: 14432921
FRACTIONATION OF THE KAPPA- AND BETA-CHAINS OF COLLAGEN BY SEPHADEX GEL FILTRATION.
Biochim Biophys Acta. 1965 Feb 15;97:366-9 PMID: 14292853

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1020148

Published

1967-01-00

Pages

148-52

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270221

Subset

External Links

PubMed source DOI: 10.1042/bj1020148 PMC Full Text View journal details

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The separation of the alpha-chains of collagen by free-flow electrophoresis.

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