Synthesis and turnover of collagen precursors in rabbit skin.

The Biochemical journal ·Vol. 102 ·No. 1 ·1967-01-00 ·Pages 143-7

Nimni ME, De Guia E, Bavetta LA

Abstract

1. The rate of synthesis of [(14)C]hydroxyproline by rabbit skin was studied in vitro and in vivo. 2. The soluble collagen fractions were shown to have a very rapid turnover. The 0.15m-sodium chloride-extractable collagen showed t((1/2)) values of 1.2hr. in vitro and 12hr. in vivo. The 0.5m-sodium chloride-extractable collagen exhibited a t((1/2)) value of 20hr. in vivo. 3. Under the conditions used it was not possible to obtain radioactive insoluble collagen in vitro. 4. A significant amount of soluble collagen is lost before it becomes insoluble. 5. These observations may help to explain why large amounts of peptide-bound hydroxyproline appear in the urine during periods of rapid collagen synthesis.

MeSH Terms

Animals Carbon Isotopes Collagen/biosynthesis Hydroxyproline/biosynthesis In Vitro Techniques Male Proline/metabolism Rabbits Skin/metabolism Sodium Chloride

Chemicals

Carbon Isotopes Sodium Chloride Collagen Proline Hydroxyproline

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Nimni M E

De Guia E

Bavetta L A

References (17)

17 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1020143

Published

1967-01-00

Pages

143-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270220

Subset

External Links

PubMed source DOI: 10.1042/bj1020143 PMC Full Text View journal details

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Synthesis and turnover of collagen precursors in rabbit skin.

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