Isolation and fractionation of rat brain nuclei.

The Journal of cell biology ·Vol. 30 ·No. 2 ·1966-08-00 ·Pages 405-15

Lovtrup-Rein H, McEwen BS

Abstract

A method for isolating pure and unaltered nuclei from rat brain by means of differential centrifugation is described. The isolated nuclei are further separated into discrete fractions of neuronal, astrocytic, and glial nuclei, with a yield amounting to 20 to 25% of the DNA of the original homogenate. Both the morphology and size of the nuclei remained unchanged. Problems concerning the composition of the isolation media, the use of detergents, as well as those raised by density gradient centrifugation in sucrose, Ficoll, and Dextran are discussed. Some values for the density of each type of brain nuclei are suggested.

MeSH Terms

Animals Brain Chemistry Cell Nucleus/analysis Centrifugation DNA/analysis Neuroglia Proteins/analysis RNA/analysis Rats

Chemicals

Proteins RNA DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Lovtrup-Rein H

McEwen B S

References (12)

12 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.30.2.405

Published

1966-08-00

Pages

405-15

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2107004

Subset

External Links

PubMed source DOI: 10.1083/jcb.30.2.405 PMC Full Text View journal details

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