Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

The Biochemical journal ·Vol. 98 ·No. 2 ·1966-02-00 ·Pages 405-9

Caygill JC, Roston CP, Jevons FR

Abstract

1. The presence of beta-galactosidase (EC 3.2.1.23) in an acetic acid extract of ram testis is reported. Some properties of the crude enzyme preparation were studied. 2. The purification of beta-acetylglucosaminase (EC 3.2.1.30) and of beta-galactosidase from the ram-testis extract by ammonium sulphate precipitation and chromatography on a CM-cellulose column is described. 3. The final purifications of the separated enzymes achieved were for the beta-acetylglucosaminase 35 times and for the beta-galactosidase 99 times. 4. The possibility of using DEAE-cellulose and Sephadex G-200 to purify the enzymes was investigated.

MeSH Terms

Animals Chemical Phenomena Chemistry Chromatography Galactosidases/metabolism Glycoside Hydrolases/metabolism Hydrogen-Ion Concentration In Vitro Techniques Male Sheep Testis/enzymology

Chemicals

Galactosidases Glycoside Hydrolases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Caygill J C

Roston C P

Jevons F R

References (8)

8 references, click to expand

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J Bacteriol. 1950 Oct;60(4):381-92 PMID: 14784466
Purification of beta-N-acetylglucosaminidase from the pig epididymis.
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Biochem J. 1961 Jan;78:111-6 PMID: 13786642
Mammalian glycosidases. 2. Properties of alpha-mannosidase and beta-galactosidase from rat epididymis.
Biochem J. 1959 Oct;73:327-34 PMID: 13811461
The attachment of carbohydrate in ovomucoid.
Biochem J. 1962 Jul;84:134-9 PMID: 13904989
[Studies on the linkage of carbohydrate to peptide in ovomucoid. I. Carbohydrate fragment obtained by baryta-hydrolysis of ovomucoid].
Yakugaku Zasshi. 1961 Oct;81:1460-3 PMID: 13919477
[Studies on the linkage of carbohydrate to peptide in ovomucoid. II. Mucopeptides obtained by pancreatic digestion].
Yakugaku Zasshi. 1961 Oct;81:1464-7 PMID: 13919478
beta-D-Fucosidase in the Limpet, Patella vulgata.
Biochem J. 1963 Apr;87:206-9 PMID: 13930238

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0980405

Published

1966-02-00

Pages

405-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1264859

Subset

External Links

PubMed source DOI: 10.1042/bj0980405 PMC Full Text View journal details

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Purification of beta-acetylglucosaminase and beta-galactosidase from ram testis.

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