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PMID: 58995 Published · ppublish English Journal Article

The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies.

The Journal of membrane biology ·Vol. 26 ·No. 4 ·1976-05-00 ·Pages 357-70

Sarkadi B, Szász I, Gárdos G

Abstract

Techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or Ca2+ by using ionophorous compounds (nigericin, gramicidin D and A 23187). Loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. After cation loading the ionophores could be bound to albumin and removed by repeated washings. The ATP and 2,3-DPG contents of the cells were practically unaltered by this treatment. Passive membrane permeability to Na+ and Ca2+ returned to normal. Loaded erythrocytes pumped out Na+ in a ouabain-sensitive and Ca2+ in a lanthanum-sensitive way. Ca2+ -loaded red cells were microspherocytes and exhibited a rapid K+ -efflux. Parallel with the extrusion of Ca2+ cells regained their biconcave shape and normal passive permeability to K+.

MeSH Terms
Adenosine Triphosphate/blood Biological Transport, Active Calcimycin/pharmacology Calcium/blood Chlorobutanol/pharmacology Diphosphoglyceric Acids/blood Erythrocytes/drug effects,metabolism,ultrastructure Gramicidin/pharmacology Humans Ionophores/pharmacology Kinetics Microscopy, Phase-Contrast Nigericin/pharmacology Oligomycins/pharmacology Ouabain/pharmacology Potassium/blood Propranolol/pharmacology Sodium/blood
Chemicals
Diphosphoglyceric Acids Ionophores Oligomycins Gramicidin Calcimycin Ouabain Adenosine Triphosphate Sodium Propranolol Chlorobutanol Nigericin Potassium Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sarkadi B
Szász I
Gárdos G
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25 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868883
Published
1976-05-00
Pages
357-70
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868883 View journal details
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