Prostaglandin: release from the rat phrenic nerve--diaphragm preparation.

Science (New York, N.Y.) ·Vol. 149 ·No. 3690 ·1965-09-17 ·Pages 1390-1

Ramwell PW, Shaw JE, Kucharski J

Abstract

Release of a substance that stimulates smooth muscle was detected from a phrenic nerve-diaphragm preparation from the rat upon direct and indirect stimulation. On thin-layer chromatography most of the active material behaved as a mixture of prostaglandins. The effect of electrical stimulation was mimicked by catecholamines but not by acetylcholine or eserine. The effect of nerve stimulation was not antagonized by d-tubocurarine.

MeSH Terms

Acetylcholine/pharmacology Animals Ascorbic Acid/pharmacology Biological Assay Bradykinin/pharmacology Chromatography, Thin Layer Diaphragm/physiology Electric Stimulation Epinephrine/pharmacology Female In Vitro Techniques Neuromuscular Junction/physiology Norepinephrine/pharmacology Phrenic Nerve/physiology Physostigmine/pharmacology Prostaglandins/metabolism Rats Uterus/drug effects

Chemicals

Prostaglandins Physostigmine Acetylcholine Ascorbic Acid Bradykinin Norepinephrine Epinephrine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Ramwell P W

Shaw J E

Kucharski J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.149.3690.1390

Published

1965-09-17

Pages

1390-1

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.149.3690.1390 View journal details

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Prostaglandin: release from the rat phrenic nerve--diaphragm preparation.

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