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PMID: 5837777 Published · ppublish English Journal Article

The linkage between the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei.

The Biochemical journal ·Vol. 96 ·No. 2 ·1965-08-00 ·Pages 302-9

Knox KW, Hall EA

Abstract

1. The linkage between the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei is rapidly hydrolysed under mild acid-hydrolysis conditions. 2. The release of the polysaccharide is accompanied by the hydrolysis of an N-acetylhexosaminide linkage. The N-acetylhexosamine residue readily forms chromogen and it is concluded that it is substituted on C((3)) by the adjacent sugar. 3. Continued heating of the polysaccharide in acid results in a slower release of reactive N-acetylhexosamine due to the hydrolysis of glycosidic linkages within the polysaccharide. 4. After the linkage between the polysaccharide and mucopeptide has been hydrolysed, acid phosphatase will release approx. 40% of the total phosphorus as inorganic phosphate. 5. It is concluded that the polysaccharide component of the cell wall is joined through its reducing end group to a phosphate grouping in the mucopeptide.

MeSH Terms
Acid Phosphatase Amino Sugars Bacterial Proteins Cell Biology Chemical Phenomena Chemistry Chemistry Techniques, Analytical Hot Temperature In Vitro Techniques Lactobacillus/cytology Mucoproteins Peptides Polysaccharides, Bacterial Sulfuric Acids
Chemicals
Amino Sugars Bacterial Proteins Mucoproteins Peptides Polysaccharides, Bacterial Sulfuric Acids Acid Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Knox K W
Hall E A
References (21)
21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0960302
Published
1965-08-00
Pages
302-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1207040
Subset
IM
External Links
PubMed source DOI: 10.1042/bj0960302 PMC Full Text View journal details
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