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PMID: 5812939 Published · ppublish English Journal Article

Pyruvate carboxylase in lactating rat and rabbit mammary gland.

The Biochemical journal ·Vol. 111 ·No. 3 ·1969-02-00 ·Pages 263-71

Gul B, Dils R

Abstract

1. Pyruvate carboxylase [pyruvate-carbon dioxide ligase (ADP), EC 6.4.1.1] was found in cell-free preparations of lactating rat and rabbit mammary glands, and optimum assay conditions for this enzyme were determined. 2. Subcellular-fractionation studies with marker enzymes showed pyruvate carboxylase to be distributed between the mitochondrial and soluble fractions of lactating rat mammary gland. Evidence is presented that the soluble enzyme is not an artifact due to mitochondrial damage. 3. In contrast, pyruvate carboxylase in lactating rabbit mammary gland is confined to the mitochondrial fraction. 4. The final product of pyruvate carboxylase action in the mitochondrial and particle-free supernatant fractions of lactating rat mammary gland was shown to be citrate. 5. The effects of freeze-drying, ultrasonic treatment and freezing-and-thawing on the specific activity of mitochondrial pyruvate carboxylase were investigated.

MeSH Terms
Animals Cell-Free System Citrates/biosynthesis Female Freeze Drying Freezing Lactation Ligases/analysis,metabolism Mammary Glands, Animal/enzymology Mitochondria/enzymology Pregnancy Pyruvates/metabolism Rabbits Rats Ultrasonics
Chemicals
Citrates Pyruvates Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gul B
Dils R
References (23)
23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1110263
Published
1969-02-00
Pages
263-71
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187507
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1110263 PMC Full Text View journal details
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