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PMID: 5812636 Published · ppublish English Journal Article

Studies on the purification of antihemophilic factor (factor 8. I. Precipitation of antihemophilic factor by concanavalin A.

The Journal of clinical investigation ·Vol. 48 ·No. 2 ·1969-02-00 ·Pages 351-8

Kass L, Ratnoff OD, Leon MA

Abstract

Concanavalin A precipitates antihemophilic factor from normal plasma. Combining this precipitation with other techniques, we were able to separate fractions rich in antihemophilic activity from human plasma with rapidity. The molecular weight of antihemophilic factor was estimated to be greater than 2 million. Presumably, antihemophilic factor is a large glycoprotein.

MeSH Terms
Animals Chemical Precipitation Electrophoresis, Disc Factor VIII/isolation & purification Glycoproteins Humans Immune Sera In Vitro Techniques Methods Molecular Weight Plant Proteins Rabbits Spectrophotometry Ultracentrifugation
Chemicals
Glycoproteins Immune Sera Plant Proteins Factor VIII
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kass L
Ratnoff O D
Leon M A
References (24)
24 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI105991
Published
1969-02-00
Pages
351-8
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC322226
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI105991 PMC Full Text View journal details
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