Formation of thiosulfate from sulfite by Desulfovibrio vulgaris.

Journal of bacteriology ·Vol. 99 ·No. 1 ·1969-07-00 ·Pages 210-5

Suh B, Akagi JM

Abstract

Crude extracts of Desulfovibrio vulgaris reduced sulfite to sulfide. Ammonium sulfate fractionation of crude extracts separated a thiosulfate-forming system from sulfite- and thiosulfate-reductase activities. Further purification by sucrose density centrifugation separated the thiosulfate-forming system into two components, both of which were required for the reaction. In addition to these two components, cytochrome c(3), ferredoxin, and hydrogenase were required to form thiosulfate from sulfite. By absorption spectra and from the effect of pH and substrate concentration, the ionic species acting as the substrate for thiosulfate-formation was concluded to be bisulfite.

MeSH Terms

Cell-Free System Desulfovibrio/enzymology,metabolism Oxidoreductases/metabolism Sulfites/metabolism Thiosulfates/metabolism

Chemicals

Sulfites Thiosulfates Oxidoreductases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Suh B

Akagi J M

References (15)

15 references, click to expand

STUDIES ON THERMOPHILIC SULFATE-REDUCING BACTERIA III. : Adenosine Triphosphate-sulfurylase of Clostridium nigrificans and Desulfovibrio desulfuricans.
J Bacteriol. 1962 Dec;84(6):1194-201 PMID: 16561978
The participation of a ferredoxin of Clostridium nigrificans in sulfite reduction.
Biochem Biophys Res Commun. 1965 Oct 8;21(1):72-7 PMID: 5865493
Cytochrome c3 and desulphoviridin; pigments of the anaerobe Desulphovibrio desulphuricans.
J Gen Microbiol. 1956 Jul;14(3):545-72 PMID: 13346018
Preparation and properties of three pure crystalline bacterial haem proteins.
Biochim Biophys Acta. 1961 Apr 1;48:266-86 PMID: 13715848
Dependance of sulfite reduction on a crystallized ferredoxin from Desulfovibrio gigas.
Biochem Biophys Res Commun. 1966 Apr 19;23(2):145-9 PMID: 5928906
Biochemical studies on sulfate-reducing bacteria. IX. Sulfite reductase.
J Biochem. 1961 Feb;49:103-9 PMID: 13718010
Rubredoxin: a new electron transfer protein from Clostridium pasteurianum.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):193-9 PMID: 5216351
Reduction of inorganic compounds with molecular hydrogen by Micrococcus lactilyticus. II. Stoichiometry with inorganic sulfur compounds.
J Bacteriol. 1962 Oct;84:659-68 PMID: 14001843
Studies on thermophilic sulfate-reducing bacteria. II. Hydrogenase activity of Clostridium nigrificans.
J Bacteriol. 1961 Dec;82:927-32 PMID: 13859876
Electron carries for the phosphoroclastic reaction of Desulfovibrio desulfuricans.
J Biol Chem. 1967 May 25;242(10):2478-83 PMID: 6026239
Inorganic chromatography.
Br Med Bull. 1954;10(3):187-92 PMID: 13199291
Evidence for the reversibility of the reaction catalyzed by adenosine 5'-phosphosulfate reductase.
Biochim Biophys Acta. 1961 May 27;49:621-4 PMID: 13733823
A colorimetric method for the determination of thiosulfate.
Biochim Biophys Acta. 1957 Feb;23(2):412-6 PMID: 13412739
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
THE ATP-DEPENDENT REDUCTION OF SULFATE WITH HYDROGEN IN EXTRACTS OF DESULFOVIBRIO DESULFURICANS.
Proc Natl Acad Sci U S A. 1959 May;45(5):701-8 PMID: 16590430

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.99.1.210-215.1969

Published

1969-07-00

Pages

210-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC249989

Subset

External Links

PubMed source DOI: 10.1128/jb.99.1.210-215.1969 PMC Full Text View journal details

Analysis Services

Formation of thiosulfate from sulfite by Desulfovibrio vulgaris.

Contact

WeChat Official Account