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PMID: 5801301 Published · ppublish English Journal Article

Agranular membranes in free polysome preparations and their possible interference in studies of protein biosynthesis.

The Biochemical journal ·Vol. 112 ·No. 3 ·1969-04-00 ·Pages 269-74

Murty CN, Hallinan T

Abstract

1. Phospholipid-rich membranous contaminants are present in free polysomes from rat liver isolated on discontinuous sucrose gradients. 2. Electron-microscopic studies indicate that the membranous contaminants are mainly agranular with very occasional granular membranes. This is confirmed by the study of their sedimentation behaviour and their initial rate of labelling with radioactive glucosamine in vivo. 3. Conventional ribosome-purification methods fail to remove the contaminants, whereas deoxycholate effectively solubilizes the membranous contaminants with little breakdown of polysomes. 4. Amino acid-incorporation studies show that these membranous contaminants may seriously interfere in assessment of the functional capacity of free polysomes in protein biosynthesis in vivo.

MeSH Terms
Animals Carbon Isotopes Centrifugation, Density Gradient Choline/metabolism Detergents Glucosamine/metabolism Glycoproteins/biosynthesis Leucine/metabolism Liver/cytology,metabolism Male Membranes/metabolism Methods Mice Microscopy, Electron Phospholipids/biosynthesis Protein Biosynthesis RNA/biosynthesis Rats Ribosomes/metabolism Starvation/metabolism Time Factors Tritium
Chemicals
Carbon Isotopes Detergents Glycoproteins Phospholipids Tritium RNA Leucine Glucosamine Choline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murty C N
Hallinan T
References (22)
22 references, click to expand
  1. Studies on the nature of polysomes.
    Biochem J. 1964 Aug;92(2):289-99 PMID: 5838072
  2. Protein synthesis by liver polyribosomes after partial hepatectomy.
    Biochem Biophys Res Commun. 1965 Jun 9;19(6):702-7 PMID: 5840696
  3. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.
    Analyst. 1966 Feb;91(79):78-88 PMID: 5323520
  4. Protein synthesis and ribonucleic acid turnover in rat-liver microsome subfractions.
    Biochim Biophys Acta. 1965 Oct 11;108(2):285-96 PMID: 5865511
  5. Studies on the protein-synthesizing activity of the ribosomes of rat liver. The activity of free polysomes.
    Biochem J. 1965 Nov;97(2):422-31 PMID: 5880015
  6. Incorporation of 14-C-choline into phospholipids of rat liver microsome subfractions: evidence against formation of endoplasmic reticulum from the nuclear membrane.
    Q J Exp Physiol Cogn Med Sci. 1966 Apr;51(2):142-50 PMID: 5178799
  7. Biosynthesis of glycoprotein by liver. The incorporation in vivo of 14C-glucosamine into protein-bound hexosamine and sialic acid of rat liver subcellular fractions.
    J Biol Chem. 1966 Nov 25;241(22):5408-18 PMID: 5954806
  8. Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.
    Biochem J. 1967 Apr;103(1):177-82 PMID: 6033756
  9. Studies on free and membrane-bound ribosomes in rat liver. II. Interaction of ribosomes and membranes.
    J Mol Biol. 1967 Jun 14;26(2):293-301 PMID: 6034776
  10. Destruction of reticulocyte ribosomes by deoxycholate.
    Biochim Biophys Acta. 1967 Sep 26;145(2):506-8 PMID: 6064639
  11. The exclusive function of reticulum bound ribosomes in glycoprotein biosynthesis.
    Life Sci. 1968 Mar 15;7(6):225-32 PMID: 5646376
  12. Early labeling with glucosamine-C14 of granular and agranular endoplasmic reticulum and free ribosomes from rat liver.
    Arch Biochem Biophys. 1968 Jun;125(3):715-20 PMID: 5671038
  13. The use of radioactive choline as a label for microsomal membranes. I. Selectivity of label for endoplasmic reticulum and specificity for lecithin.
    Biochim Biophys Acta. 1968 Sep 17;163(2):218-25 PMID: 5686279
  14. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  15. Glycogen synthesis from uridine diphosphate glucose. The distribution of the enzyme in liver cell fractions.
    J Biophys Biochem Cytol. 1961 Jun;10:195-209 PMID: 13764028
  16. Ribonucleoprotein from a rat tumor, the Jensen sarcoma. III. Ribosomes purified without deoxycholate but with bentonite as ribonuclease inhibitor.
    J Biol Chem. 1963 Jan;238:318-23 PMID: 13942717
  17. The isolation of microsomal membranes.
    Biochem Soc Symp. 1963;22:4-31 PMID: 13975385
  18. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.
    Nature. 1963 Feb 2;197:430-5 PMID: 14000153
  19. PREPARATION OF RAT-LIVER POLYSOMES WITHOUT THE UTILIZATION OF DETERGENT.
    Biochim Biophys Acta. 1964 May 18;87:177-80 PMID: 14171039
  20. POLYRIBOSOMES IN RAT TISSUES. I. A STUDY OF IN VIVO PATTERNS IN LIVER AND HEPATOMAS.
    Cancer Res. 1964 Aug;24:1229-37 PMID: 14216156
  21. The estimation of glycogen with the anthrone reagent.
    Arch Biochem. 1950 Jan;25(1):191-200 PMID: 15401229
  22. BIOSYNTHESIS OF GLYCOPROTEINS. IV. THE SUBCELLULAR SITES OF INCORPORATION OF GLUCOSAMINE-1-14-C INTO GLYCOPROTEIN RAT LIVER.
    J Biol Chem. 1965 May;240:1882-8 PMID: 14299605
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1120269
Published
1969-04-00
Pages
269-74
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187705
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1120269 PMC Full Text View journal details
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