Abstract
The mediated transport of citrate in Aerobacter aerogenes was studied. According to data obtained by examining the distribution of radioactive citrate at room temperature and at 0 C, a carrier system appears to be located on the membrane. The carrier system is inducible and very specific, not acting on the related compounds isocitrate and cis-aconitate. Induction required synthesis of both ribonucleic acid and protein as determined by starving auxotrophic mutants and by using specific inhibitors of protein synthesis. Citrate transport was inhibited by N-ethyl maleimide, dinitrofluorobenzene, and uranyl nitrate. A kinetic study of uranyl nitrate inhibition revealed that the inhibition of citrate transport was different from that of glucose penetration. Cyanide also discriminated citrate from glucose penetration inhibiting only the former. These last results suggested that energy is required for citrate penetration.
MeSH Terms
Azides/pharmacology
Bacterial Proteins/biosynthesis
Biological Transport
Carbon Isotopes
Chloramphenicol/pharmacology
Citrates/metabolism,pharmacology
Cyanides/pharmacology
Dinitrophenols/pharmacology
Electrophoresis
Enterobacter/drug effects,enzymology,metabolism
Enzyme Induction
Ethylmaleimide/pharmacology
Isocitrate Dehydrogenase/metabolism
Membrane Transport Proteins/metabolism
Uracil/pharmacology
Chemicals
Azides
Bacterial Proteins
Carbon Isotopes
Citrates
Cyanides
Dinitrophenols
Membrane Transport Proteins
Uracil
Chloramphenicol
Isocitrate Dehydrogenase
Ethylmaleimide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Villarreal-Moguel E I
Ruiz-Herrera J
References (17)
17 references, click to expand
-
THE ROLE OF PERMEASE IN TRANSPORT.
Biochim Biophys Acta. 1964 Jan 27;79:177-200
PMID: 14114517
-
Citrate transport system of Streptococcus diacetilactis.
J Bacteriol. 1962 May;83:1005-9
PMID: 13905110
-
A binding site for sulfate and its relation to sulfate transport into Salmonella typhimurium.
J Biol Chem. 1966 Sep 10;241(17):3962-9
PMID: 5331786
-
Genetic regulatory mechanisms in the synthesis of proteins.
J Mol Biol. 1961 Jun;3:318-56
PMID: 13718526
-
Evidence for the occurrence of Permeases for tricarboxylic acid cycle intermediates in Pseudomonas aeruginosa.
J Gen Microbiol. 1959 Feb;20(1):144-55
PMID: 13631191
-
USE OF URANYL ION IN MEMBRANE TRANSPORT STUDIES.
J Bacteriol. 1964 Jan;87:232-3
PMID: 14102863
-
Inhibition by puromycin of amino acid transport by embryonic chick bone.
Biochim Biophys Acta. 1966 Feb 28;115(2):355-60
PMID: 5943439
-
Tricarboxylic acid cycle in Pseudomonas aeruginosa.
J Biol Chem. 1951 Jun;190(2):853-8
PMID: 14841238
-
Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli.
Proc Natl Acad Sci U S A. 1965 Sep;54(3):891-9
PMID: 5324399
-
GENETIC CONTROL OF THE MEMBRANE PROTEIN COMPONENT OF THE LACTOSE TRANSPORT SYSTEM OF Escherichia coli.
Proc Natl Acad Sci U S A. 1967 Mar;57(3):698-705
PMID: 16591520
-
Oxidative pathways in a fluorescent Pseudomonas.
Biochem J. 1953 Dec;55(5):800-11
PMID: 13115376
-
The nature of the adaptive lag of Pseudomonas fluorescens toward citrate.
J Bacteriol. 1953 Feb;65(2):187-92
PMID: 13034714
-
Amino acid transport in embryonic chick bone and rat costal cartilage.
Biochim Biophys Acta. 1966 Feb 28;115(2):345-54
PMID: 5943438
-
THE ROLES OF INDUCER AND CATABOLITE REPRESSOR IN THE SYNTHESIS OF BETA-GALACTOSIDASE BY ESCHERICHIA COLI.
J Mol Biol. 1964 Jan;8:105-27
PMID: 14149954
-
A colorimetric method for the assay of soluble succinic dehydrogenase and pyridinenucleotide-linked dehydrogenases.
Arch Biochem Biophys. 1959 Dec;85:561-2
PMID: 13820076
-
Catabolite repression.
Cold Spring Harb Symp Quant Biol. 1961;26:249-56
PMID: 14468226
-
Inhibition of amino Acid transport in rat kidney cortex by puromycin.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):371-8
PMID: 16591480