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PMID: 5775687 Published · ppublish English Journal Article

Preparation and some properties of L-3-glycerophosphate dehydrogenase from pig brain mitochondria.

The Biochemical journal ·Vol. 111 ·No. 1 ·1969-01-00 ·Pages 27-34

Dawson AP, Thorne CJ

Abstract

1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A study was made of the effects of various analogues of l-3-glycerophosphate on the activity of the enzyme.

MeSH Terms
Animals Brain/enzymology Electron Transport Flavins/analysis Glycerolphosphate Dehydrogenase/analysis,isolation & purification Glycerophosphates/metabolism In Vitro Techniques Iron/analysis Mitochondria/enzymology Swine
Chemicals
Flavins Glycerophosphates Iron Glycerolphosphate Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dawson A P
Thorne C J
References (17)
17 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1969-01-00
Pages
27-34
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1187490
Subset
IM
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