Effective specific activity dilution in labeled DNA of cultured mammalian cells (L5178Y).

Biophysical journal ·Vol. 9 ·No. 2 ·1969-02-00 ·Pages 122-6

Burki HJ, Okada S

Abstract

Upon labeling the DNA of cultured mammalian cells with radio-active thymidine it was found that there was a large reduction in the specific activity of radioactive thymidine in DNA when this specific activity was compared to the specific activity of the exogenous supply. This reduction in specific activity may be determined once the effective dilution factor for thymidine in mammalian cells is known. The average effective dilution factor was found to be 2 x 10(-4) M for L5178Y cells in log growth.

MeSH Terms

Animals Carbon Isotopes Culture Techniques DNA/biosynthesis Leukemia, Experimental Mice Thymidine/metabolism Time Factors Tritium

Chemicals

Carbon Isotopes Tritium DNA Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Burki H J

Okada S

References (6)

6 references, click to expand

Determination of thymidine metabolism in HeLa cell cultures by a combined electrophoretic and paper chromatographic method.
Exp Cell Res. 1966 Nov-Dec;44(2):645-8 PMID: 5964774
DEVELOPMENT, MAINTENANCE AND ASSAY OF DRUG RESISTANCE.
Methods Med Res. 1964;10:247-62 PMID: 14284928
STUDIES ON THE IN VITRO INCORPORATION OF TRITIATED THYMIDINE INTO ASCITES TUMOR CELLS.
Exp Cell Res. 1963 Aug;31:390-6 PMID: 14065129
LIMITATIONS IN THE USE OF CARBON-LABELED AND TRITIUM-LABELED THYMIDINE IN CELL CULTURE STUDIES.
Radiat Res. 1964 Mar;21:355-66 PMID: 14131651
Studies of the culture of leukemic cells in vitro.
Ann N Y Acad Sci. 1958 Dec 5;76(3):673-80 PMID: 13627893
Effects of temperature on growth rate of cultured mammalian cells (L5178Y).
J Cell Biol. 1967 Feb;32(2):309-23 PMID: 10976224

Article Info

Journal

Biophysical journal

Abbr.

Biophys J

ISSN

0006-3495

DOI

10.1016/S0006-3495(69)86373-3

Published

1969-02-00

Pages

122-6

Language

English

Region

United States

NLM ID

0370626

PMCID

PMC1367421

Subset

External Links

PubMed source DOI: 10.1016/S0006-3495(69)86373-3 PMC Full Text View journal details

Analysis Services

Effective specific activity dilution in labeled DNA of cultured mammalian cells (L5178Y).

Contact

WeChat Official Account