Characterization of the tumorlike (T) antigen induced by type 12 adenovirus. I. Purification of the antigen from infected KB cells and a hamster tumor cell line.

Journal of virology ·Vol. 2 ·No. 1 ·1968-01-00 ·Pages 7-14

Gilead Z, Ginsberg HS

Abstract

The T antigen induced by type 12 adenovirus was purified from KB cells infected in the presence of 10(-6)m 5-fluoro-2-deoxyuridine to inhibit synthesis of viral capsid antigens. The antigen was purified approximately 200-fold, and the purified product contained only negligible amounts of host-cell contaminants, as judged by the residual radioactivity from (14)C-labeled uninfected cells which had been added to infected cells at the initiation of the purification. Immunoelectrophoresis indicated that the purified T-antigen preparation contained a single antigenic species. The T antigen from a hamster cell line (HT-1) derived from a type 12 adenovirus-induced tumor was purified by the same procedure. The T antigens from the two different sources were shown to be immunologically similar by use of a rabbit antiserum prepared against the purified T antigen from infected KB cells and sera from hamsters bearing tumors induced by type 12 adenovirus.

MeSH Terms

Adenoviridae/drug effects,immunology Animals Antigens/analysis,isolation & purification Carbon Isotopes Carcinoma/immunology Cell Line Chromatography Complement Fixation Tests Cricetinae Culture Techniques Deoxyuridine/pharmacology Immunoelectrophoresis Mouth Neoplasms/immunology Neoplasms/immunology Rabbits

Chemicals

Antigens Carbon Isotopes Deoxyuridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gilead Z

Ginsberg H S

References (26)

26 references, click to expand

Comparison of the rates of ultraviolet inactivation of the capacity of type 12 adenovirus to infect cells and to induce T antigen formation.
J Bacteriol. 1966 Dec;92(6):1853-4 PMID: 5958120
IMMUNOFLUORESCENT STUDIES OF ADENOVIRUS 12 TUMORS AND OF CELLS TRANSFORMED OR INFECTED BY ADENOVIRUSES.
J Exp Med. 1964 Oct 1;120:577-88 PMID: 14212121
Adenovirus 12 virion-free "T" antigen from infected cells inhibited with cytosine arabinoside.
Nature. 1966 Jun 25;210(5043):1381-3 PMID: 5963775
PRODUCTION OF "TUMOR-SPECIFIC" ANTIGENS BY ONCOGENIC VIRUSES DURING ACUTE CYTOLYTIC INFECTIONS.
Proc Natl Acad Sci U S A. 1965 Jan;53:12-9 PMID: 14283190
Immunofluorescence of human adenovirus type 12 in various cell types.
Proc Soc Exp Biol Med. 1966 Feb;121(2):405-14 PMID: 4956667
Propagation in a fluid medium of a human epidermoid carcinoma, strain KB.
Proc Soc Exp Biol Med. 1955 Jul;89(3):362-4 PMID: 13254761
Inhibition of adenovirus replication by 1-beta-D-arabinofuranosylcytosine.
Proc Soc Exp Biol Med. 1966 May;122(1):243-7 PMID: 5329457
Characterization of a Tumorlike Antigen in Type 12 and Type 18 Adenovirus-Infected Cells.
J Bacteriol. 1965 Jul;90(1):120-5 PMID: 16562005
TUMOR IMMUNITY IN HAMSTERS INFECTED WITH ADENOVIRUS TYPE 12 OR SIMIAN VIRUS 40.
Proc Soc Exp Biol Med. 1964 Nov;117:575-9 PMID: 14233501
Application of a microtechnique to viral serological investigations.
J Immunol. 1962 Mar;88:320-9 PMID: 13910995
A new and rapid method for isolating viruses by selective fluorocarbon deproteinization.
Trans N Y Acad Sci. 1956 Jun;18(8):701-3 PMID: 13352364
Studies of adenovirus SV40 hybrid viruses. I. Assay system and further evidence for hybridization.
J Exp Med. 1965 Nov 1;122(5):943-54 PMID: 4285252
Electron microscopic localization of virus-associated antigens in human amnion cells (AV-3) infected with human adenovirus, type 12.
Virology. 1966 Apr;28(4):751-4 PMID: 4287207
Detection of adenovirus type 12 neoantigen (s) in a continuous human amnion cell line (FL) by immunofluorescence.
Proc Soc Exp Biol Med. 1965 Dec;120(3):832-7 PMID: 5323559
SPECIFIC ADENOVIRUS COMPLEMENT-FIXING ANTIGENS IN VIRUS-FREE HAMSTER AND RAT TUMORS.
Proc Natl Acad Sci U S A. 1963 Aug;50:379-89 PMID: 14060660
Etiologic relationship of the RI-67 agent to acute respiratory disease (ARD).
J Clin Invest. 1955 Jun;34(6):820-31 PMID: 14381511
Amino acid metabolism in mammalian cell cultures.
Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
A micro-electrophoretic method.
Nature. 1958 Mar 22;181(4612):839-40 PMID: 13526686
Virus-induced transplantation immunity to human adenovirus type 12 tumors of the hamster and mouse.
Proc Soc Exp Biol Med. 1966 Apr;121(4):1216-9 PMID: 4287123
Specific transplantation antigens of mouse sarcomas induced by adenovirus type 12.
J Exp Med. 1967 Apr 1;125(4):689-701 PMID: 6020008
CHARACTERISTICS OF HUMAN ADENOVIRUS TYPE 12 INDUCED HAMSTER TUMOR CELLS IN TISSUE CULTURE.
Proc Soc Exp Biol Med. 1964 Jul;116:563-8 PMID: 14194601
Immunoelectrophoresis with cellulose acetate.
Nature. 1962 Apr 14;194:185-6 PMID: 13902431
Intracellular uncoating of type 5 adenovirus deoxyribonucleic acid.
J Virol. 1967 Oct;1(5):851-67 PMID: 5621482
Synthesis of virus-specific polymers in adenovirus-infected cells; effect of 5-fluorodeoxyuridine.
J Exp Med. 1962 Aug 1;116:141-57 PMID: 13893423
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Properties of cells derived from adenovirus-induced hamster tumors by long-term in vitro cultivation. I. Clonal stability of three biological characteristics.
Virology. 1966 Apr;28(4):633-44 PMID: 5940378

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.2.1.7-14.1968

Published

1968-01-00

Pages

7-14

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC375571

Subset

External Links

PubMed source DOI: 10.1128/JVI.2.1.7-14.1968 PMC Full Text View journal details

Analysis Services

Characterization of the tumorlike (T) antigen induced by type 12 adenovirus. I. Purification of the antigen from infected KB cells and a hamster tumor cell line.

Contact

WeChat Official Account