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PMID: 5739847 Published · ppublish English Journal Article

Genetic relatedness studies with adenovirus-associated viruses.

Journal of virology ·Vol. 2 ·No. 10 ·1968-10-00 ·Pages 999-1005

Rose JA, Hoggan MD, Koczot F, Shatkin AJ

Abstract

Adenovirus-associated viruses (AAV) contain double-stranded deoxyribonucleic acid (DNA). DNA from each of the four AAV serotypes was used as template for the in vitro synthesis of complementary (3)H-ribonucleic acids(RNA). An estimation of genetic interrelatedness was made on the basis of hybridization reactions between synthetic AAV RNA and AAV DNA. Heterologous reactions were 27 to 37% of homologous reactions, suggesting that the AAV serotypes are related to about the same extent. AAV-1 synthetic RNA was also reacted with DNA from helper adenovirus types 2, 7, and SV15. Very low levels of RNA binding were observed, but it is not likely that these reactions represent AAV-adenovirus genetic relatedness.

MeSH Terms
Adenoviridae/classification Antigens Culture Techniques DNA, Viral Genetics, Microbial Hybridization, Genetic RNA, Viral/biosynthesis Satellite Viruses Serotyping Templates, Genetic Tritium
Chemicals
Antigens DNA, Viral RNA, Viral Tritium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Rose J A
Hoggan M D
Koczot F
Shatkin A J
References (23)
23 references, click to expand
  1. Role of DNA in RNA synthesis. XI. Selective transcription of gamma DNA segments in vitro by RNA polymerase of Escherichia coli.
    J Mol Biol. 1967 May 28;26(1):19-38 PMID: 5341412
  2. Genetic differentiation by nucleic Acid homology I. Relationships among Mycoplasma species of man.
    J Bacteriol. 1966 Aug;92(2):302-10 PMID: 16562111
  3. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
    J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
  4. Genetic Differentiation by Nucleic Acid Homology II. Genotypic Variations Within Two Mycoplasma Species.
    J Bacteriol. 1966 Aug;92(2):311-7 PMID: 16562112
  5. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus.
    Virology. 1963 May;20:199-207 PMID: 13950097
  6. Nucleic acid from an adeno-associated virus: chemical and physical studies.
    Proc Natl Acad Sci U S A. 1966 Jul;56(1):86-92 PMID: 5229859
  7. Physicochemical characterization of adeno-associated satellite virus type 4 and its nucleic acid.
    J Virol. 1967 Oct;1(5):980-7 PMID: 4987175
  8. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467-74 PMID: 5227666
  9. THE PURIFICATION OF SIMIAN VIRUS 40.
    Virology. 1964 Nov;24:381-7 PMID: 14227039
  10. Physical assay and growth cycle studies of a defective adeno-satellite virus.
    J Virol. 1967 Feb;1(1):171-80 PMID: 4990036
  11. Selective synthesis of T-even bacteriophage early messenger in vitro.
    J Mol Biol. 1966 Aug;19(2):541-7 PMID: 5338857
  12. Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.
    Proc Natl Acad Sci U S A. 1962 Jan 15;48:81-94 PMID: 13877961
  13. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  14. RNA production in adenovirus-infected KB cells.
    Virology. 1965 Dec;27(4):571-9 PMID: 5855573
  15. Stimulation of DNA synthesis in mouse cell line 3T3 by Simian virus 40.
    Proc Natl Acad Sci U S A. 1966 Oct;56(4):1170-6 PMID: 4291201
  16. Sedimentation characteristics of rapidly labelled RNA from HeLa cells.
    Biochem Biophys Res Commun. 1962 Jun 4;7:486-90 PMID: 14498283
  17. Isolation of adenovirus-associated viruses from man.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1410-5 PMID: 4295829
  18. Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
    J Mol Biol. 1962 Jul;5:109-18 PMID: 14470099
  19. Properties of a small virus associated with adenovirus type 4.
    J Immunol. 1966 Dec;97(6):754-66 PMID: 5955169
  20. Serologic evidence for human infection with adenovirus-associated viruses.
    J Natl Cancer Inst. 1968 Feb;40(2):319-27 PMID: 4295610
  21. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles.
    Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509-15 PMID: 4291405
  22. Genetic relatedness among mycoplasmas as determined by nucleic acid homology.
    J Bacteriol. 1966 Jan;91(1):153-60 PMID: 5903090
  23. ADENOVIRUS-ASSOCIATED DEFECTIVE VIRUS PARTICLES.
    Science. 1965 Aug 13;149(3685):754-6 PMID: 14325163
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.2.10.999-1005.1968
Published
1968-10-00
Pages
999-1005
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375430
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.2.10.999-1005.1968 PMC Full Text View journal details
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