Semi-automated method for the differential determination of plasma catecholamines.

Journal of clinical pathology ·Vol. 21 ·No. 6 ·1968-11-00 ·Pages 759-63

McCullough H

Abstract

A method is described for the estimation of adrenaline and noradrenaline in a 5 to 10 ml plasma sample.A batch technique of adsorption and elution from alumina is used and the final estimation is carried out using an automated fluorimetric technique based on the trihydroxyindole method. Fluorescence is stabilized first by thioglycollic acid to give noradrenaline concentrations only and then by ascorbic acid to give the total catecholamine concentration. The results of a number of catecholamine analyses are given.

MeSH Terms

Adsorption Adult Aluminum Ascorbic Acid Autoanalysis Epinephrine/blood Fluorometry Humans Male Methods Norepinephrine/blood Pheochromocytoma/blood Thioglycolates Urinary Bladder Neoplasms/blood

Chemicals

Thioglycolates Aluminum Ascorbic Acid Norepinephrine Epinephrine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

McCullough H

References (8)

8 references, click to expand

An automated trihydroxyindole procedure for the differential analysis of catecholamines.
Clin Chem. 1965 Nov;11(11):986-97 PMID: 5847986
An improved semi-automated procedure for fluorometric determination of plasma catecholamines.
Clin Chim Acta. 1965 Aug;12(2):191-7 PMID: 5856282
A new and sensitive bioassay for catecholamines.
J Pharm Pharmacol. 1965 Sep;17(9):589-93 PMID: 4379634
Survey of chemical and physical methods for measuring catecholamines.
Pharmacol Rev. 1959 Jun;11(2, Part 2):252-5 PMID: 13667401
A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines.
J Pharmacol Exp Ther. 1962 Dec;138:360-75 PMID: 14013351
An autoanalytical method for the estimation of adrenaline and noradrenaline.
Nature. 1962 Mar 10;193:988 PMID: 14472888
The fluorimetric estimation of catecholamines.
Methods Med Res. 1961;9:130-46 PMID: 14040207
Sympatho-adrenal responses to general anesthesia in man and their relation to hemodynamics.
Anesthesiology. 1959 Sep-Oct;20:563-75 PMID: 14434847

Article Info

Journal

Journal of clinical pathology

Abbr.

J Clin Pathol

ISSN

0021-9746

DOI

10.1136/jcp.21.6.759

Published

1968-11-00

Pages

759-63

Language

English

Region

England

NLM ID

0376601

PMCID

PMC473956

Subset

External Links

PubMed source DOI: 10.1136/jcp.21.6.759 PMC Full Text View journal details

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Semi-automated method for the differential determination of plasma catecholamines.

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