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PMID: 570212 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The induction of macrophage spreading by factor B of the properdin system.

The Journal of experimental medicine ·Vol. 149 ·No. 2 ·1979-02-01 ·Pages 372-86

Götze O, Bianco C, Cohn ZA

Abstract

Unstimulated mouse peritoneal macrophages attached to a glass substratum responded to activated human factor B (Bb) of the properdin system but not to native factor B with rapid spreading and a concomitant increase in their apparent surface area. Excellent correlation of the distribution of Bb protein and cell-spreading activity was found upon purification of Bb by ion-exchange and molecular seive chromatography and alkaline polyacrylamide gel electrophoresis. 1.6 microgram of purified Bb was sufficient to induce spreading in 50% of 5 x 10(4) glass attached macrophages within 1-2 h at 37 degrees C. Treatment of Bb with di-isopropyl-fluorophosphate indicated that the intact catalytic site of the serine-proteinase Bb was required for the initiation of macrophage spreading. The involvement of factor B in the induction of rapid cell spreading could also be indirectly demonstrated in an autologous system in which F(ab')2 fragments of an antiserum to mouse B prevented mouse macrophages from spreading in response to complement-activated mouse serum. These experiments suggest a role for factor B and the alternative pathway of complement fixation in the localization of mononuclear phagocytes to areas of inflammation.

MeSH Terms
Animals Ascitic Fluid/immunology Binding Sites Cell Adhesion/drug effects Cell Movement/drug effects Complement Factor B/antagonists & inhibitors,immunology,pharmacology Dose-Response Relationship, Drug Enzyme Precursors/immunology Humans Macrophages/immunology Mice Molecular Weight Peptide Fragments/pharmacology Species Specificity
Chemicals
Enzyme Precursors Peptide Fragments Complement Factor B
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Götze O
Bianco C
Cohn Z A
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32 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.149.2.372
Published
1979-02-01
Pages
372-86
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2184799
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.149.2.372 PMC Full Text View journal details
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