Affinity site labeling of a mouse myeloma protein which binds dinitrophenyl ligands.

Science (New York, N.Y.) ·Vol. 162 ·No. 3860 ·1968-12-20 ·Pages 1398-400

Metzger H, Potter M

Abstract

A mouse myeloma protein of the immunoglobulin A class, which specifically binds dinitrophenyl ligands, has been successfully affinity labeled (site-directed labeling) with several diazonium reagents, leading to inactivation of the combining sites. The labeling reagents reacted only with tyrosine residues of light chain origin. The 7S subunit of the myeloma protein appears to contain only one reactive site.

MeSH Terms

Animals Binding Sites Boric Acids Caproates Chelating Agents Diazonium Compounds Dinitrophenols Immunochemistry Immunoglobulin G Indicators and Reagents Mice Multiple Myeloma/immunology Neoplasm Proteins Nitrobenzenes Peptides Spectrum Analysis

Chemicals

Boric Acids Caproates Chelating Agents Diazonium Compounds Dinitrophenols Immunoglobulin G Indicators and Reagents Neoplasm Proteins Nitrobenzenes Peptides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Metzger H

Potter M

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.162.3860.1398

Published

1968-12-20

Pages

1398-400

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.162.3860.1398 View journal details

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Affinity site labeling of a mouse myeloma protein which binds dinitrophenyl ligands.

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