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PMID: 5688079 Published · ppublish English Journal Article

Cellular differentiation and the aging process in cartilaginous tissues. Mucopolysaccharide synthesis in cell cultures of chondrocytes.

The Journal of experimental medicine ·Vol. 128 ·No. 6 ·1968-12-01 ·Pages 1353-62

Shulman HJ, Meyer K

Abstract

Primary cell cultures of differentiated chondrocytes were shown to produce chondroitin-4-sulfate as the predominant mucopolysaccharide, with suggestive evidence for the synthesis of keratan sulfate and possibly chondroitin-6-sulfate. Chicken embryonic cartilage was shown to be composed mainly of chondroitin-4-sulfate, with a small amount of chondroitin-6-sulfate, but essentially no keratan sulfate. These findings were compared to the data of others, and a hypothesis explaining the aging process in cartilage in terms of cellular differentiation was presented.

MeSH Terms
Animals Cartilage/cytology,embryology Cell Differentiation Chick Embryo Chondroitin/analysis,biosynthesis Culture Techniques Electrophoresis Infrared Rays Spectrum Analysis Sulfates/analysis
Chemicals
Sulfates Chondroitin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shulman H J
Meyer K
References (34)
34 references, click to expand
  1. DIFFERENCES IN GLYCOSAMINOGLYCANS DERIVED FROM CHICK EMBRYO CHONDROCYTES GROWN IN VITRO AND IN VIVO.
    Biochim Biophys Acta. 1964 Mar 2;83:61-8 PMID: 14152199
  2. The loss of phenotypic traits by differentiated cells. IV. Changes in polysaccharides produced by dividing chondrocytes.
    Dev Biol. 1967 Sep;16(3):250-81 PMID: 6056993
  3. Production of chondroitin sulfate in tissue culture of cartilage.
    Biochim Biophys Acta. 1963 Jul 16;74:193-7 PMID: 13950809
  4. Infra-red spectroscopic studies of some polysaccharides.
    Biochim Biophys Acta. 1954 Jun;14(2):173-81 PMID: 13172234
  5. Mucopolysaccharides of costal cartilage.
    Science. 1958 Oct 17;128(3329):896 PMID: 13592269
  6. THE LOSS OF PHENOTYPIC TRAITS BY DIFFERENTIATED CELLS IN VITRO, I. DEDIFFERENTIATION OF CARTILAGE CELLS.
    Proc Natl Acad Sci U S A. 1960 Dec;46(12):1533-42 PMID: 16590779
  7. Infra-red spectra of carbohydrate sulphate esters.
    Nature. 1959 Aug 15;184(Suppl 8):548-9 PMID: 14417716
  8. Fractionation of acid mucopolysaccharides on DEAE-Sephadex anion exchanger.
    Biochim Biophys Acta. 1962 Sep 24;63:346-8 PMID: 13992024
  9. Isomeric chondroitin sulphates.
    Nature. 1958 Feb 8;181(4606):421-2 PMID: 13504223
  10. THE FORM AND FUNCTION OF SYNOVIAL CELLS IN TISSUE CULTURES : II. THE PRODUCTION OF MUCIN.
    J Exp Med. 1933 Jun 30;58(1):85-95 PMID: 19870183
  11. The acid mucopolysaccharides of connective tissue.
    Biochim Biophys Acta. 1956 Sep;21(3):506-18 PMID: 13363957
  12. The estimation of glycogen with the anthrone reagent.
    Arch Biochem. 1950 Jan;25(1):191-200 PMID: 15401229
  13. Differentiation in vitro: effects of Sephadex fractions of chick embryo extract.
    Science. 1966 Sep 2;153(3740):1116-9 PMID: 5918574
  14. Production of mucopolysaccharides by synovial cells in a simplified tissue culture medium.
    Proc Soc Exp Biol Med. 1957 Jan;94(1):51-6 PMID: 13400868
  15. The loss of phenotypic traits by differentiated cells. 3. The reversible behavior of chondrocytes in primary cultures.
    J Cell Biol. 1966 Mar;28(3):473-87 PMID: 4163861
  16. Clonal stability and phenotypic expression of chick cartilage cells in vitro.
    Proc Natl Acad Sci U S A. 1966 Jan;55(1):66-73 PMID: 5328643
  17. The surface properties of cancer cells: a review.
    Cancer Res. 1962 Jun;22:525-48 PMID: 13858936
  18. Mucopolysaccharides produced in tissue culture.
    J Biophys Biochem Cytol. 1957 May 25;3(3):391-6 PMID: 13438923
  19. The mucopolysaccharides of bovine cornea.
    J Biol Chem. 1953 Dec;205(2):611-6 PMID: 13129238
  20. PRODUCTION OF HYALURONATE AND COLLAGEN BY FIBROBLAST CLONES IN CULTURE.
    Nature. 1964 Feb 15;201:710 PMID: 14134721
  21. Acid mucopolysaccharide patterns in aging human cartilage.
    J Clin Invest. 1966 Jul;45(7):1103-11 PMID: 4226136
  22. Differentiation of fibroblasts in tissue culture, as determined by mucopolysaccharide production.
    Proc Soc Exp Biol Med. 1955 Jan;88(1):31-5 PMID: 14357335
  23. The binding of cations by chondroitin sulfate.
    J Clin Invest. 1957 Dec;36(12):1715-22 PMID: 13491703
  24. Production of hyaluronic acid in tissue culture of Rous sarcoma.
    Nature. 1962 Nov 24;196:782-3 PMID: 13950810
  25. Quantitative studies on the production of acid mucopolysaccharides by replicate cell cultures of rat fibroblasts.
    Ann N Y Acad Sci. 1960 Jun 30;86:878-915 PMID: 13772817
  26. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  27. Plaque formation and isolation of pure lines with poliomyelitis viruses.
    J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
  28. Proteinpolysaccharide of bovine cartilage. I. Extraction and electrophoretic studies.
    J Biol Chem. 1967 Sep 10;242(17):3799-804 PMID: 4227022
  29. The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1144-51 PMID: 5240020
  30. Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.
    J Biol Chem. 1968 Apr 10;243(7):1536-42 PMID: 4231029
  31. The acid mucopolysaccharides of connective tissue. III. the sulfate linkage.
    Biochim Biophys Acta. 1958 Oct;30(1):184-5 PMID: 13584412
  32. CONTROL OF CHONDROGENESIS IN THE EMBRYO.
    Biophys J. 1964 Jan;4:SUPPL239-55 PMID: 14104078
  33. Study of the time-course production of acid mucopolysaccharides by fibroblasts in a synthetic medium.
    Anat Rec. 1958 Dec;132(4):585-96 PMID: 13650188
  34. Ageing of human cartilage.
    Nature. 1959 May 2;183(4670):1267-8 PMID: 13657080
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.128.6.1353
Published
1968-12-01
Pages
1353-62
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138571
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.128.6.1353 PMC Full Text View journal details
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