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PMID: 5685862 Published · ppublish English Journal Article

Choline acetyltransferase binding to and release from membranes.

The Biochemical journal ·Vol. 109 ·No. 3 ·1968-09-00 ·Pages 389-98

Fonnum F

Abstract

1. The binding of non-occluded choline acetyltransferase to synaptosome membranes is a reversible process that is primarily dependent on the pH and ionic strength of the suspending medium. 2. The distribution of soluble enzyme bound to synaptosome membranes was studied by density-gradient centrifuging. 3. Choline acetyltransferase shows enzyme activity both in the free and in the membrane-bound form. 4. Varying the temperature or prolonged hypo-osmotic treatment does not release the membrane-bound enzyme. 5. The release of choline acetyltransferase from membranes by different anions, thiols, adenosine nucleotides and enzyme substrates was studied.

MeSH Terms
Acyltransferases/metabolism Animals Binding Sites Brain/enzymology Centrifugation, Density Gradient Hot Temperature Hydrogen-Ion Concentration Hypotonic Solutions Membranes Rats Synapses/enzymology Time Factors
Chemicals
Hypotonic Solutions Acyltransferases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fonnum F
References (21)
21 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1090389
Published
1968-09-00
Pages
389-98
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186833
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1090389 PMC Full Text View journal details
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