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PMID: 5667526 Published · ppublish English Journal Article

Centrifugation of mammalian cells on gradients: a new rotor.

Science (New York, N.Y.) ·Vol. 161 ·No. 3844 ·1968-08-30 ·Pages 911-3

Pretlow TG, Boone CW

Abstract

A short-arm rotor increases separation of viable mammalian cells, from mixtures, by low-speed centrifugation; continuous Ficoll density gradients in tissue-culture media are used. We describe the theory and experimental demonstration of the superior separation achieved with this new rotor.

MeSH Terms
Animals Carcinoma, Ehrlich Tumor Cell Biology/instrumentation Centrifugation, Density Gradient/instrumentation Culture Media HeLa Cells Mammals Methods Polysaccharides Rotation
Chemicals
Culture Media Polysaccharides
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pretlow T G
Boone C W
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.161.3844.911
Published
1968-08-30
Pages
911-3
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.161.3844.911 View journal details
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