Nucleotide composition of nuclear and mitochondrial deoxyribonucleic acid of fungi.

Journal of bacteriology ·Vol. 96 ·No. 1 ·1968-07-00 ·Pages 184-90

Villa VD, Storck R

Abstract

The buoyant density of nuclear and mitochondrial deoxyribonucleic acid (DNA) from 14 species of fungi was determined by CsCl density gradient equilibrium centrifugation. The buoyant density of both types of DNA was the same for all three Mucorales analyzed. The buoyant density of mitochondrial DNA was significantly lower than that of the nuclear DNA for nine species of Ascomycetes and two species of Basidiomycetes. No simple correlation could be obtained from the comparison of the two types of DNA. Mitochondrial DNA represented a very small proportion of total DNA. Heat-denatured mitochondrial DNA renatured more readily than nuclear DNA.

MeSH Terms

Ascomycota/analysis Basidiomycota/analysis Centrifugation, Density Gradient Cytosine Nucleotides/analysis DNA/analysis Densitometry Fungi/analysis Guanine Nucleotides/analysis Mitochondria/analysis Neurospora/analysis

Chemicals

Cytosine Nucleotides Guanine Nucleotides DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Villa V D

Storck R

References (29)

29 references, click to expand

Mitochondrial-satellite and circular DNA filaments in yeast.
Science. 1967 Jun 2;156(3779):1234-7 PMID: 6025542
The isolation of deoxyribonucleic acid from lamb heart mitochondria.
J Biol Chem. 1966 Mar 10;241(5):1019-22 PMID: 5933860
Isolation of deoxyribonucleic acid from mitochondria of chick embryo heart and liver.
Proc Natl Acad Sci U S A. 1965 May;53(5):1126-33 PMID: 5222555
Nutrition, growth, and morphogenesis of Mucor rouxii.
J Bacteriol. 1962 Oct;84:841-58 PMID: 13969720
Circular DNA filaments from mouse mitochondria.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):508-14 PMID: 5229973
Separation and characterization of mitochondrial DNA from yeast.
Biochem Biophys Res Commun. 1965 Oct 26;21(2):141-8 PMID: 5863857
Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
Nucleotide composition of nucleic acids of fungi. II. Deoxyribonucleic acids.
J Bacteriol. 1966 Jan;91(1):227-30 PMID: 5948191
Mitochondrial DNA synthesis and the mitotic cycle in Physarum polycephalum.
Biochim Biophys Acta. 1967 Jun 20;142(1):181-94 PMID: 6069137
Evidence for the mitochondrial origin of frog egg cytoplasmic DNA.
Proc Natl Acad Sci U S A. 1966 Jul;56(1):269-76 PMID: 5229852
Synthesis of a cytoplasmic DNA during the G2 interphase of Physarum polycephalum.
Biochem Biophys Res Commun. 1966 Mar 22;22(6):678-83 PMID: 5949776
The DNA of chloroplasts, mitochondria and centrioles.
Prog Nucleic Acid Res Mol Biol. 1967;6:143-86 PMID: 5338004
Mitochondrial deoxyribonucleic acid of Tetrahymena. Its partial physical characterization.
Biochemistry. 1966 Jul;5(7):2214-21 PMID: 5959448
Physiological variations in satellite components of yeast DNA detected by density gradient centrifugation.
Biochem Biophys Res Commun. 1966 Apr 6;23(1):56-61 PMID: 5928893
UNIQUENESS OF BACTERIAL RIBOSOMES.
Proc Natl Acad Sci U S A. 1964 Oct;52:958-65 PMID: 14224400
Mitochondrial DNA from yeast "petite" mutants: specific changes in buoyant density corresponding to different cytoplasmic mutations.
Biochem Biophys Res Commun. 1966 Jul 20;24(2):218-24 PMID: 5965229
DNA IN MITOCHONDRIA OF NEUROSPORA CRASSA.
Proc Natl Acad Sci U S A. 1964 Oct;52:931-8 PMID: 14224397
Mitochondrial and cytoplasmic polysomes from Neurospora crassa.
Nature. 1967 Sep 23;215(5108):1340-5 PMID: 6055444
Mitochondrial DNA in yeast and some mammalian species.
Science. 1966 Feb 11;151(3711):687-9 PMID: 5908073
Isolation and characterization of DNA from the mitochondrial fraction of Euglena.
J Mol Biol. 1966 Jun;17(2):463-9 PMID: 5963078
Nearest neighbor frequencies and the phylogenetic origin of mitochondrial DNA in Physarum polycephalum.
J Mol Biol. 1967 Jan 28;23(2):281-4 PMID: 6067777
The isolation and some properties of rat liver mitochondrial deoxyribonucleic acid.
Proc Natl Acad Sci U S A. 1965 Dec;54(6):1650-8 PMID: 5218918
Sedimentation coefficients of RNA from 70S and 80S ribosomes.
Proc Natl Acad Sci U S A. 1967 Jan;57(1):164-9 PMID: 4963253
Inhibition by cycloheximide of protein and RNA synthesis in Mucor rouxii.
Biochem Biophys Res Commun. 1966 Jan 24;22(2):175-80 PMID: 5902292
POLYMER SIMILAR TO POLYDEOXYADENYLATE-THYMIDYLATE IN VARIOUS TISSUES OF A MARINE CRAB.
Science. 1964 Mar 27;143(3613):1442-3 PMID: 14107446
Replication and inheritance of mitochondrial DNA.
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1600-8 PMID: 5227678
Ribosomal RNA and ribosomes from mitochondria of Neurospora crassa.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1025-32 PMID: 5233834
EQUILIBRIUM SEDIMENTATION OF MACROMOLECULES IN DENSITY GRADIENTS.
Proc Natl Acad Sci U S A. 1957 Jul 15;43(7):581-8 PMID: 16590059
Mitochondiral DNA from protozoa.
Genetics. 1965 Nov;52(5):1051-8 PMID: 5884410

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.96.1.184-190.1968

Published

1968-07-00

Pages

184-90

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252271

Subset

External Links

PubMed source DOI: 10.1128/jb.96.1.184-190.1968 PMC Full Text View journal details

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Nucleotide composition of nuclear and mitochondrial deoxyribonucleic acid of fungi.

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