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PMID: 5640378 Published · ppublish English Journal Article

N-methyl groups in bacterial lipids. 3. Phospholipids of hyphomicrobia.

Journal of bacteriology ·Vol. 95 ·No. 2 ·1968-02-00 ·Pages 367-75

Goldfine H, Hagen P

Abstract

The phospholipids of Hyphomicrobium vulgare NQ-521 have been separated by preparative thin-layer chromatography and analyzed by paper chromotography of the water-soluble products of acid and mild alkaline hydrolysis. The principal phospholipids are phosphatidyl ethanolamine (23%), phosphatidyl N,N'-dimethylethanolamine (36%), lecithin (29%), and phosphatidyl glycerol (10%). Three other strains of Hyphomicrobium were found to have similar phospholipid compositions. Growing cells incorporated the methyl group of methionine into lipid-bound N,N'-dimethylethanolamine and choline. Experiments with sonic extracts of H. vulgare NQ-521 and (14)C (methyl) S-adenosylmethionine demonstrated the formation of phosphatidyl N-monomethylethanolamine in addition to the dimethylethanolamine and choline phosphatides.

MeSH Terms
Bacteria/analysis Carbon Isotopes Chromatography, Paper Chromatography, Thin Layer Lipids/analysis Methionine/metabolism Methylation Phosphatidylcholines/analysis Phosphatidylethanolamines/analysis Phospholipids/analysis
Chemicals
Carbon Isotopes Lipids Phosphatidylcholines Phosphatidylethanolamines Phospholipids Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goldfine H
Hagen P
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24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.95.2.367-375.1968
Published
1968-02-00
Pages
367-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC252028
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.95.2.367-375.1968 PMC Full Text View journal details
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