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PMID: 5638887 Published · ppublish English Journal Article

The fine structure of puromycin-induced changes in mouse entorhinal cortex.

The Journal of cell biology ·Vol. 36 ·No. 2 ·1968-02-00 ·Pages 379-90

Gambetti P, Gonatas NK, Flexner LB

Abstract

Bitemporal intracerebral injections of puromycin in mice suppress indefinitely expression of memory of avoidance-discrimination learning. Ultrastructural studies of the entorhinal cortex of puromycin-treated mice revealed the following: (a) Abnormalities were not observed in presynaptic terminals and synaptic clefts; many postsynaptic dendrites or somas contained swollen mitochondria. (b) Dispersion of polyribosomes into single units or condensation of ribosomes into irregular aggregates with loss of "distinctiveness" was noted in a few neurons 7-27 hr after puromycin treatment. (c) Cytoplasmic aggregates of granular or amorphous material were frequently noted within otherwise normal neuronal perikarya. (d) Mitochondria in many neuronal perikarya and dendrites were swollen. Mitochondria in axons, presynaptic terminals, and glial cells were unaltered. The relationships between these lesions and the effect of puromycin on protein synthesis and memory are examined. It is suggested that the disaggregation of polysomes is too limited to explain the effect of puromycin on memory. Special emphasis is given to the swelling of mitochondria. The possible mechanisms and the significance of this lesion are discussed.

MeSH Terms
Animals Avoidance Learning/drug effects Axons Behavior, Animal/drug effects Cell Aggregation Cerebral Cortex/cytology,drug effects Cytoplasm Dendrites Discrimination Learning/drug effects Injections Memory/drug effects Mice Microscopy, Electron Mitochondria/drug effects Puromycin/pharmacology Ribosomes Synapses/drug effects
Chemicals
Puromycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gambetti P
Gonatas N K
Flexner L B
References (31)
31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.36.2.379
Published
1968-02-00
Pages
379-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107358
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.36.2.379 PMC Full Text View journal details
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