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PMID: 562889 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Histogenesis of mouse cerebellum in microwell cultures. Cell reaggregation and migration, fiber and synapse formation.

The Journal of cell biology ·Vol. 75 ·No. 3 ·1977-12-00 ·Pages 915-40

Trenkner E, Sidman RL

Abstract

A microwell culture system was developed for analysis of cell movements and interactions during nervous system histogenesis. Cells from trypsinized 7-day-old mouse cerebellum reaggregated within hours into clusters which later developed interconnections consisting of either sheets of migrating cells and cell processes or cables of fiber bundles with cells migrating along their surfaces. Granule cells in several stages of differentiation, basket and/or stellate neurons, some larger neurons, and two types of neuroglial cells were identified in reproducible, nonrandom patterns by scanning and transmission electron microscopy. Axonal and dendritic processes, both with growth cones, and numerous synapses were generated in vitro.

MeSH Terms
Animals Axons/ultrastructure Cell Aggregation Cell Differentiation Cell Movement Cells, Cultured Cerebellum/cytology Dendrites/ultrastructure Mice Morphogenesis Nerve Fibers/growth & development Neuroglia/ultrastructure Neurons/cytology Synapses
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Trenkner E
Sidman R L
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41 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.75.3.915
Published
1977-12-00
Pages
915-40
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111581
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.75.3.915 PMC Full Text View journal details
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