Specific interactions of distamycin A and its analogs with (A-T) rich and (G-C) rich duplex regions of DNA and deoxypolynucleotides.

Nucleic acids research ·Vol. 4 ·No. 8 ·1977-08-00 ·Pages 2655-70

Luck G, Zimmer C, Reinert KE, Arcamone F

Abstract

The specific interaction of distamycin A and analogs with DNA's and synthetic deoxypolynucleotide duplexes were studied in detail by means of circular dichroism and the data were analyzed together with viscosity results of several natural DNA's. At low ligand to nucleotide ratio the previously reported specific binding to (A-T) pairs of DNA is verified by a highly favoured interaction with (A-T)-enriched segments of distamycins containing four and five methylpyrrole carboxamide units. At higher distamycin concentration a second specific binding to (G-C) pairs most probably through hydrogen bonding is established. Viscometric results suggest a distamycin-induced local bending of the helix and could support the idea of a preferential alignment of the ligand molecule along only one strand in the groove which differs from the netropsin interaction mechanism. The possibility of an overlapping binding of the oligopeptides in the small groove is discussed.

MeSH Terms

Animals Cattle Chemical Phenomena Chemistry Circular Dichroism DNA DNA, Bacterial Distamycins Molecular Weight Nucleic Acid Conformation Polydeoxyribonucleotides Pyrroles Structure-Activity Relationship Thymus Gland Viscosity

Chemicals

DNA, Bacterial Distamycins Polydeoxyribonucleotides Pyrroles DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Luck G

Zimmer C

Reinert K E

Arcamone F

References (21)

21 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.8.2655

Published

1977-08-00

Pages

2655-70

Language

English

Region

England

NLM ID

0411011

PMCID

PMC342599

Subset

External Links

PubMed source DOI: 10.1093/nar/4.8.2655 PMC Full Text View journal details

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Specific interactions of distamycin A and its analogs with (A-T) rich and (G-C) rich duplex regions of DNA and deoxypolynucleotides.

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