Homogeneous cell populations from rabbit kidney cortex. Proximal, distal tubule, and renin-active cell isolated by free-flow electrophoresis.
A single-cell suspension has been prepared from rabbit kidney cortex by using a Ca-binding medium and gentle mechanical forces. The suspension was subjected to carrier-free electrophoresis, and several cell fractions were obtained. Proximal and distal tubule cell populations could be identified by their morphology. Renin-containing cells were located by means of radioimmunoassay. The morphology of the cells and their vitality (uridine incorporation) are discussed.
17 references, click to expand
-
A new method for the preparation of liver cell suspensions.Exp Cell Res. 1962 Sep;27:453-67 PMID: 13964289
-
Preparative free-flow electrophoresis for the isolation of membrane, organelle and cell fractions from rabbit kidney cortex.Curr Probl Clin Biochem. 1976;6:108-12 PMID: 1001001
-
Isolation of renal tubules.Methods Enzymol. 1974;32:658-64 PMID: 4374636
-
Preparation of kidney cell suspensions and respiration of kidney and liver cell suspensions.Exp Cell Res. 1970 Dec;63(2):333-40 PMID: 4321428
-
Subcellular localization of renin and kallikrein in rat kidney.Ital J Biochem. 1975 May-Aug;24(3-4):199-206 PMID: 1213875
-
Sites of enzyme activity along the nephron.Kidney Int. 1976 Mar;9(3):233-42 PMID: 940267
-
Application of a radioimmunoassay for angiotensin I to the physiologic measurements of plasma renin activity in normal human subjects.J Clin Endocrinol Metab. 1969 Oct;29(10):1349-55 PMID: 4311082
-
Isolation and subfractionation on ficoll gradients of adult rat hepatocytes. Size, morphology, and biochemical characteristics of cell fractions.J Cell Biol. 1975 Jul;66(1):1-22 PMID: 167028
-
Criteria of viability of isolated liver cells.Hoppe Seylers Z Physiol Chem. 1975 Jun;356(6):827-38 PMID: 241693
-
[An enzymatic method for the isolation of tubules and cells from human kidney cortex].Z Klin Chem Klin Biochem. 1975 Jan;13(1):31-5 PMID: 164742
-
The large-scale isolation of renin-containing granules from rabbit renal cortex by zonal centrifugation.J Lab Clin Med. 1975 Mar;85(3):467-77 PMID: 163876
-
Dispersion and cultivation of renal cells after short-term storage of kidneys.J Clin Microbiol. 1975 Sep;2(3):243-6 PMID: 170318
-
Preparation of renal papillary collecting duct cells for study in vitro.Kidney Int. 1975 Sep;8(3):191-4 PMID: 170449
-
Isolation of renin granules from rat kidney cortex and evidence for an inactive form of renin (prorenin) in granules and plasma.Endocrinology. 1976 Jun;98(6):1466-74 PMID: 1278112
-
Control of cell growth. IV. Growth properties of a new cell line established from an estrogen-dependent kidney tumor of the Syrian hamster.Endocrinology. 1976 May;98(5):1260-72 PMID: 1261519
-
Distribution of renin in subcellular fractions from the rabbit kidney.Jpn J Pharmacol. 1975 Jun;25(3):295-301 PMID: 1185902
-
In vitro culture of cells isolate from dimethylnitrosamine-induced renal mesenchymal tumors of the rat. I. Qualitative morphology.J Natl Cancer Inst. 1975 May;54(5):1085-95 PMID: 1127737
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