Haem a, cytochrome c and total protein turnover in mitochondria from rat heart and liver.

The Biochemical journal ·Vol. 119 ·No. 2 ·1970-09-00 ·Pages 157-60

Aschenbrenner B, Druyan R, Albin R, Rabinowitz M

Abstract

Haem a and cytochrome c were isotopically labelled in mitochondria from rat heart and liver after injection of delta-amino[2,3-(3)H(2)]laevulate, a specific haem precursor. [guanido-(14)C]Arginine or l-[4,5-(3)H(2)]leucine were used to label mitochondrial proteins. Half-lives were measured from biological decay in vivo and were similar (5.5-6.2 days) for haem a, cytochrome c and [(14)C]arginine-labelled proteins. Labelling of hepatic mitochondrial proteins with [(3)H(2)]leucine resulted in a prolonged apparent half-life.

MeSH Terms

Amino Acids/metabolism Animals Arginine/metabolism Carbon Isotopes Cytochromes/metabolism Female Heme/metabolism Leucine/metabolism Levulinic Acids/metabolism Mitochondria, Liver/metabolism Mitochondria, Muscle/metabolism Myocardium/metabolism Proteins/metabolism Rats Tritium

Chemicals

Amino Acids Carbon Isotopes Cytochromes Levulinic Acids Proteins Tritium Heme Arginine Leucine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Aschenbrenner B

Druyan R

Albin R

Rabinowitz M

References (17)

17 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1190157

Published

1970-09-00

Pages

157-60

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1179336

Subset

External Links

PubMed source DOI: 10.1042/bj1190157 PMC Full Text View journal details

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Haem a, cytochrome c and total protein turnover in mitochondria from rat heart and liver.

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