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PMID: 5480850 Published · ppublish English Journal Article

Effects of glucagon on adenosine 3',5'-monophosphate and guanosine 3',5'-monophosphate in human plasma and urine.

The Journal of clinical investigation ·Vol. 49 ·No. 12 ·1970-12-00 ·Pages 2237-45

Broadus AE, Kaminsky NI, Northcutt RC, Hardman JG, Sutherland EW, Liddle GW

Abstract

Glucagon, infused intravenously into fasting, well-hydrated, normal men in doses of 25-200 ng/kg per min, induced up to 30-fold increases in both plasma and urinary cyclic AMP. Cyclic GMP levels were unaffected by glucagon. Simultaneous cyclic AMP and inulin clearance studies demonstrated that the glucagon-induced increase in urinary cyclic AMP was entirely due to glomerular filtration of the elevated plasma levels of the nucleotide. The cyclic AMP response to glucagon was not mediated by parathyroid hormone or epinephrine, and trypsintreated glucagon was completely inactive. The perfused rat liver released cyclic AMP into the perfusate in response to glucagon, indicating that the liver is a possible source of the cyclic AMP entering the extracellular fluids in response to glucagon in vivo.

MeSH Terms
Adenine Nucleotides/blood,metabolism,urine Adult Epinephrine/pharmacology Fasting Glucagon/pharmacology Glucose/pharmacology Guanine Nucleotides/blood,metabolism,urine Humans Injections, Intravenous Insulin/pharmacology Liver/drug effects,metabolism Male Parathyroid Hormone/pharmacology Perfusion Trypsin/pharmacology Trypsin Inhibitors/pharmacology
Chemicals
Adenine Nucleotides Guanine Nucleotides Insulin Parathyroid Hormone Trypsin Inhibitors Glucagon Trypsin Glucose Epinephrine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Broadus A E
Kaminsky N I
Northcutt R C
Hardman J G
Sutherland E W
Liddle G W
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37 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI106442
Published
1970-12-00
Pages
2237-45
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC322724
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI106442 PMC Full Text View journal details
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