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PMID: 5472154 Published · ppublish English Journal Article

A radiochemical assay for glutamine synthetase, and activity of the enzyme in rat tissues.

The Biochemical journal ·Vol. 118 ·No. 1 ·1970-06-00 ·Pages 35-9

Lund P

Abstract

1. A radiochemical assay for glutamine synthetase has been developed in which an ATP-regenerating system is incorporated to prevent accumulation of inhibitory amounts of ADP. It is particularly suitable for assay of the enzyme in crude tissue extracts containing high adenosine triphosphatase activity. 2. A survey of the distribution of the enzyme in tissues from normal male rats showed that activity is present in liver, brain cortex, kidney cortex, spleen, testis and retina. 3. The K(m) of the enzyme for l-glutamate is approx. 1.5x10(-2)m.

MeSH Terms
Adenine Nucleotides Adenosine Triphosphate Ammonia Animals Brain/enzymology Carbon Isotopes Glutamates Glutamine Kidney/enzymology Ligases/analysis Liver/enzymology Male Methods Radiochemistry Rats Retina/enzymology Spleen/enzymology Testis/enzymology
Chemicals
Adenine Nucleotides Carbon Isotopes Glutamates Glutamine Ammonia Adenosine Triphosphate Ligases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lund P
References (13)
13 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1180035
Published
1970-06-00
Pages
35-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179075
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1180035 PMC Full Text View journal details
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