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PMID: 5458991 Published · ppublish English Journal Article

New observations on microbodies. A cytochemical study on CPIB-treated rat liver.

The Journal of cell biology ·Vol. 45 ·No. 1 ·1970-04-00 ·Pages 118-29

Legg PG, Wood RL

Abstract

The liver of male rats has been studied after CPIB stimulation by using the peroxidase reaction for localizing catalase in hepatic cells. CPIB administration leads to an increase in the number of microbodies, and it is suggested that one mechanism by which microbody proliferation occurs is a process of fragmentation or budding from preexisting microbodies. Reaction product was observed not only within the microbody matrix, but outside the limiting membrane of the microbody and in association with ribosomes of adjacent rough endoplasmic reticulum. This localization of reaction product is interpreted as evidence that catalase after synthesis on rough endoplasmic reticulum may accumulate near microbodies and may be transferred directly into these organelles without traversing the cisternae of the endoplasmic reticulum or Golgi apparatus.

MeSH Terms
Animals Anticholesteremic Agents/pharmacology Clofibrate/pharmacology Endoplasmic Reticulum Golgi Apparatus Histocytochemistry Liver/cytology,drug effects,enzymology Male Mitochondria Peroxidases/metabolism Propionates/pharmacology Rats Ribosomes
Chemicals
Anticholesteremic Agents Propionates Peroxidases Clofibrate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Legg P G
Wood R L
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32 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.45.1.118
Published
1970-04-00
Pages
118-29
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107995
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.45.1.118 PMC Full Text View journal details
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