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PMID: 54354 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Ultrastructural study of Mycoplasma pneumoniae in organ culture.

Journal of bacteriology ·Vol. 125 ·No. 1 ·1976-01-00 ·Pages 332-9

Wilson MH, Collier AM

Abstract

The ultrastructure of Mycoplasma pneumoniae M129 was studied by using specialized staining methods for thin-section transmission electron microscopy. Nucleic acid was shown in the cytoplasmic granules and fibrillar material in the nuclear region. The central filament of the highly structured tip contained basic protein. With one method of fixation, parallel filaments were seen in the central core. M. pneumoniae was enveloped in an extracellular mucoprotein layer that was especially concentrated around its terminal structure.

MeSH Terms
Animals Bacterial Proteins/isolation & purification Cricetinae Cytoplasmic Granules/ultrastructure DNA, Bacterial/isolation & purification Glycoproteins/isolation & purification Hydrolyzable Tannins Indium Mucoproteins/isolation & purification Mycoplasma/ultrastructure Organ Culture Techniques Phosphotungstic Acid Ruthenium Red Staining and Labeling Trachea
Chemicals
Bacterial Proteins DNA, Bacterial Glycoproteins Hydrolyzable Tannins Mucoproteins Indium Ruthenium Red Phosphotungstic Acid
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson M H
Collier A M
References (11)
11 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.125.1.332-339.1976
Published
1976-01-00
Pages
332-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233367
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.125.1.332-339.1976 PMC Full Text View journal details
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