Home LiteratureArticle Details
PMID: 5413328 Published · ppublish English Journal Article

Association of lactoferrin with specific granules in rabbit heterophil leukocytes.

The Journal of experimental medicine ·Vol. 131 ·No. 3 ·1970-03-01 ·Pages 559-70

Baggiolini M, De Duve C, Masson PL, Heremans JF

Abstract

Lactoferrin has been identified in rabbit heterophil leukocytes on the basis of its immunological reactivity, electrophoretic mobility, acid-resistant iron-binding properties, and spectral characteristics. Leukocyte lactoferrin was found to be exclusively localized in the specific (secondary) granules, which have been resolved from other subcellular components by zonal differential centrifugation and by isopycnic equilibration.

MeSH Terms
Animals Cytoplasmic Granules/analysis Electrophoresis Iron/metabolism Leukocytes/analysis,metabolism Protein Binding Proteins/analysis Rabbits
Chemicals
Proteins Iron
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Baggiolini M
De Duve C
Masson P L
Heremans J F
References (21)
21 references, click to expand
  1. Immunochemical quantitation of antigens by single radial immunodiffusion.
    Immunochemistry. 1965 Sep;2(3):235-54 PMID: 4956917
  2. Origin of granules in polymorphonuclear leukocytes. Two types derived from opposite faces of the Golgi complex in developing granulocytes.
    J Cell Biol. 1966 Feb;28(2):277-301 PMID: 5914694
  3. Fine structural studies on the development of heterophil, eosinophil, and basophil granulocytes in rabbits.
    Lab Invest. 1967 Mar;16(3):349-82 PMID: 4164826
  4. Immunohistochemical localization and bacteriostatic properties of an iron-binding protein from bronchial mucus.
    Thorax. 1966 Nov;21(6):538-44 PMID: 5339630
  5. Fine structural localization of acid and alkaline phosphatases in cells of rabbit blood and bone marrow.
    J Histochem Cytochem. 1967 Jun;15(6):311-34 PMID: 4292606
  6. Arginine-rich proteins of polymorphonuclear leukocyte lysosomes. Antimicrobial specificity and biochemical heterogeneity.
    J Exp Med. 1968 May 1;127(5):927-41 PMID: 4871738
  7. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions.
    J Cell Biol. 1968 May;37(2):482-513 PMID: 4297786
  8. Isolation and characterization of rabbit serum and milk transferrins. Evidence for difference in sialic acid content only.
    Biochemistry. 1968 Apr;7(4):1371-8 PMID: 5677827
  9. Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. I. Histochemical staining of bone marrow smears.
    J Cell Biol. 1968 Nov;39(2):286-98 PMID: 4878049
  10. Differences in enzyme content of azurophil and specific granules of polymorphonuclear leukocytes. II. Cytochemistry and electron microscopy of bone marrow cells.
    J Cell Biol. 1968 Nov;39(2):299-317 PMID: 5692583
  11. Resolution of granules from rabbit heterophil leukocytes into distinct populations by zonal sedimentation.
    J Cell Biol. 1969 Feb;40(2):529-41 PMID: 5812474
  12. Metal-combining properties of human lactoferrin (red milk protein). 1. The involvement of bicarbonate in the reaction.
    Eur J Biochem. 1968 Dec 5;6(4):579-84 PMID: 5701973
  13. Inhibition of bacteria by lactoferrin and other iron-chelating agents.
    Biochim Biophys Acta. 1968 Dec 23;170(2):351-65 PMID: 4974829
  14. Lactoferrin, an iron-binding protein in neutrophilic leukocytes.
    J Exp Med. 1969 Sep 1;130(3):643-58 PMID: 4979954
  15. The influence of phagocytosis on the intracellular distribution of granule-associated components of polymorphonuclear leucocytes.
    J Exp Med. 1960 Dec 1;112:1015-22 PMID: 13694489
  16. Degranulation of polymorphonuclear leucocytes following phagocytosis of microorganisms.
    J Exp Med. 1960 Dec 1;112:1005-14 PMID: 13714579
  17. [Preparation and properties of lactosiderophilin (lactotransferrin) of human milk].
    Biochim Biophys Acta. 1960 Dec 18;45:413-21 PMID: 13772242
  18. [Isolation and characterization of the red siderophilic protein from maternal milk: lactotransferrin].
    Bull Soc Chim Biol (Paris). 1961;43:929-43 PMID: 13869779
  19. HISTOCHEMICAL DISTRIBUTION OF LYSOZYME ACTIVITY IN ORGANS OF NORMAL MICE AND RADIATION CHIMERAS.
    J Histochem Cytochem. 1964 May;12:384-91 PMID: 14193859
  20. ELECTRON MICROSCOPE STUDIES ON THE DEGRANULATION OF RABBIT PERITONEAL LEUKOCYTES DURING PHAGOCYTOSIS.
    J Exp Med. 1964 Oct 1;120:569-76 PMID: 14212120
  21. Phagocytin: a bactericidal substance from polymorphonuclear leucocytes.
    J Exp Med. 1956 May 1;103(5):589-611 PMID: 13319580
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.131.3.559
Published
1970-03-01
Pages
559-70
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138819
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.131.3.559 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2