Mitochondrial malic enzyme in mosaic skeletal muscle of mouse chimeras.

Biochemical genetics ·Vol. 17 ·No. 7-8 ·1979-08-00 ·Pages 693-702

Frair PM, Strasberg PM, Freeman KB, Peterson AC

Abstract

The question was investigated whether mitochondria in the mammalian skeletal muscle fiber syncytium incorporate gene products encoded by one or many nuclei. Mouse chimeras were produced from strains which differ in their electrophoretic variants of the nuclear-coded mitochondrial protein, malic enzyme (MOD-2, E.C. 1.1.1.40, L-malate NADP+ oxidoreductase decarboxylating). The MOD-2 phenotypes of skeletal muscles of these chimeras were characterized in a starch gel electrophoretic system. The results indicate that individual mitochondria can contain products encoded by multiple nuclei and therefore that, for skeletal muscle mitochondria, the cell is not subdivided into nuclear territories. Possible mechanisms of gene product distribution in skeletal muscle fibers are discussed.

MeSH Terms

Animals Chimera Electrophoresis, Starch Gel Genes Malate Dehydrogenase/genetics Mice Mice, Inbred C57BL/genetics Mice, Inbred Strains/genetics Mitochondria, Muscle/enzymology Muscles/enzymology Myocardium/enzymology Phenotype

Chemicals

Malate Dehydrogenase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Frair P M

Strasberg P M

Freeman K B

Peterson A C

References (24)

24 references, click to expand

Isozymes and genetic control of NADP-malate dehydrogenase in mice.
Arch Biochem Biophys. 1966 Oct;117(1):28-33 PMID: 4382005
Clonal origins of somites and their muscle derivatives: evidence from allophenic mice.
Dev Biol. 1972 Sep;29(1):27-37 PMID: 5074211
Chimaera mouse study shows absence of disease in genetically dystrophic muscle.
Nature. 1974 Apr 12;248(449):561-4 PMID: 4824025
Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids.
Ann Hum Genet. 1975 Oct;39(2):203-12 PMID: 1088824
Synthesis, transfer, and specific binding of purified L-( 35 S)methionine-labeled rat liver mitochondrial adenosine triphosphatase and its subunits to mitochondrial inner membrane.
Biochemistry. 1972 Aug 15;11(17):3154-62 PMID: 4261764
Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey Macaca nemestrina.
Biochem Genet. 1972 Dec;7(3):289-301 PMID: 4630448
Functions for polyribosome-membrane interactions in protein synthesis.
Biochim Biophys Acta. 1977 Aug 9;472(2):197-236 PMID: 329880
Subunit structure and gene control of mouse NADP-malate dehydrogenase.
Biochem Genet. 1969 Jan;2(4):351-60 PMID: 5770240
Studies of the selective permeation of radioactively labelled aspartate aminotransferase isozymes into mitochondria in vitro.
Eur J Biochem. 1978 Feb;83(2):427-35 PMID: 631127
Transport of cytoplasmically synthesized proteins into the mitochondria in a cell free system from Neurospora crassa.
Eur J Biochem. 1977 Dec;81(3):533-44 PMID: 145943
Relationship of genotype and degree of chimerism in coat color to sex ratios and gametogenesis in chimeric mice.
J Exp Zool. 1971 Oct;178(2):165-76 PMID: 5165469
Genetic control of mitochondrial malic enzyme in mouse brain.
J Biol Chem. 1979 Jan 10;254(1):83-7 PMID: 758327
Cytoplasmic type 80 S ribosomes associated with yeast mitochondria. II. Evidence for the association of cytoplasmic ribosomes with the outer mitochondrial membrane in situ.
J Biol Chem. 1974 May 25;249(10):3297-303 PMID: 4598123
Mitochondrial malate dehydrogenase and malic enzyme: Mendelian inherited electrophoretic variants in the mouse.
Biochem Genet. 1970 Dec;4(6):707-18 PMID: 5496232
A technique for detection and relative quantitative analysis of glucosephosphate isomerase isozymes from nanogram tissue samples.
Biochem Genet. 1978 Aug;16(7-8):681-90 PMID: 728061
Charge properties of mitochondrial matrix proteins.
Can J Biochem. 1975 Nov;53(11):1150-7 PMID: 1192257
Comparison of mitochondrial components from human and hamster cell lines.
Exp Cell Res. 1974 Dec;89(2):399-401 PMID: 4477074
Studies on isolated human skeletal muscle fibers, including a proposed pattern of nuclear distribution and a concept of nuclear territories.
Hum Pathol. 1974 Jul;5(4):441-61 PMID: 4275783
Normal mammalian muscle differentiation and gene control of isocitrate dehydrogenase synthesis.
Proc Natl Acad Sci U S A. 1967 Aug;58(2):592-8 PMID: 5233460
A cytochrome-related inherited disorder of the nervous system and muscle.
Arch Neurol. 1970 Aug;23(2):103-12 PMID: 5430330
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Selective permeability of rat liver mitochondria to purified aspartate aminotransferases in vitro.
Biochem J. 1977 Jun 15;164(3):685-91 PMID: 883959
Newly synthesized acetylcholine receptors are located in the Golgi apparatus.
J Cell Biol. 1978 Jan;76(1):237-44 PMID: 563402
Kinetic studies on the transport of cytoplasmically synthesized proteins into the mitochondria in intact cells of Neurospora crassa.
Eur J Biochem. 1977 Dec;81(3):523-32 PMID: 202461

Article Info

Journal

Biochemical genetics

Abbr.

Biochem Genet

ISSN

0006-2928

DOI

10.1007/BF00502127

Published

1979-08-00

Pages

693-702

Language

English

Region

United States

NLM ID

0126611

Subset

External Links

PubMed source DOI: 10.1007/BF00502127 View journal details

Analysis Services

Mitochondrial malic enzyme in mosaic skeletal muscle of mouse chimeras.

Contact

WeChat Official Account