Demonstration of Y/autosomal translocations using distamycin A.

Human genetics ·Vol. 53 ·No. 1 ·1979-00-00 ·Pages 107-9

Schmid M

Abstract

The condensation of the brightly fluorescing Y-heterochromatin is prevented by cultivating leukocytes in a medium containing distamycin A. This technique provides a reliable method for the identification of those Y/autosomal translocations in which Y heterochromatin is involved. A case of a familiar Y/22 translocation and the distamycin A technique are described.

MeSH Terms

Chromosomes, Human, 21-22 and Y Culture Media Distamycins Female Heterochromatin Humans Leukocytes/ultrastructure Male Pyrroles Sex Chromosomes Translocation, Genetic Y Chromosome/drug effects

Chemicals

Culture Media Distamycins Heterochromatin Pyrroles

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Schmid M

References (10)

10 references, click to expand

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Ann Genet. 1978 Dec;21(4):239-42 PMID: 314264
An azoospermic male with a Y/autosome translocation.
Hum Genet. 1979 Jan 25;46(2):155-8 PMID: 422199
Nonhomologous associations of C-heterochromatin at human male meitoic prophase.
Cytogenet Cell Genet. 1979;23(1-2):23-32 PMID: 761482
A case of a reciprocal translocation between the Y and no. 1 chromosomes.
Jinrui Idengaku Zasshi. 1978 Sep;23(3):225-31 PMID: 732018
[A satellited Y chromosome].
Ann Genet. 1978 Dec;21(4):237-8 PMID: 314263
Visualization of nucleolar organizer regions im mammalian chromosomes using silver staining.
Chromosoma. 1975 Nov 20;53(1):37-50 PMID: 53131
Identification of human chromosomes by DNA-binding fluorescent agents.
Chromosoma. 1970;30(2):215-27 PMID: 4193398
Effects of distamycin A on human leukocytes in vitro.
Cytogenet Cell Genet. 1979;23(1-2):103-7 PMID: 83927
A simple technique for demonstrating centromeric heterochromatin.
Exp Cell Res. 1972 Nov;75(1):304-6 PMID: 4117921
Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining.
Chromosoma. 1979 Feb 21;71(2):197-216 PMID: 85513

Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00289459

Published

1979-00-00

Pages

107-9

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00289459 View journal details

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Demonstration of Y/autosomal translocations using distamycin A.

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