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PMID: 5344147 Published · ppublish English Journal Article

Electron microscopic radioautographic detection of sites of protein synthesis and migration in liver.

The Journal of cell biology ·Vol. 43 ·No. 2 ·1969-11-00 ·Pages 237-49

Ashley CA, Peters T

Abstract

The sites of synthesis of proteins and their subsequent migration in rat liver have been studied during a 75 min period after labeling of liver-slice proteins by exposure to leucine-H(3) for 2 min. Incorporation of the label into protein began after 1 min and was maximal by 4 min. Electron microscopic radioautography showed that synthesis of proteins in hepatocytes occurs mainly on ribosomes, particularly those in rough endoplasmic reticulum and, to some extent, in nuclei and mitochondria. Most of the newly formed proteins leave the endoplasmic reticulum in the course of 40 min, and concurrently labeled proteins appear in Golgi bodies, smooth membranes, microbodies, and lysosomes. A likely pathway for the secretion of some or all plasma proteins is from typical rough endoplasmic reticulum to a zone of reticulum which is partially coated with ribosomes, to the Golgi apparatus, and thence to the cell periphery. The formation of protein by reticuloendothelial cells was measured and found to be about 5% of the total protein formed by the liver.

MeSH Terms
Animals Antimetabolites Autoradiography Cell Nucleus/metabolism Cytoplasm/metabolism Emulsions Endoplasmic Reticulum/metabolism Golgi Apparatus/metabolism Leucine/metabolism Liver/cytology,metabolism Lysosomes/metabolism Membranes/metabolism Microscopy, Electron Protein Biosynthesis Proteins/metabolism Puromycin/pharmacology Rats Reticulocytes/metabolism Ribosomes/metabolism Time Factors Tritium
Chemicals
Antimetabolites Emulsions Proteins Tritium Puromycin Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ashley C A
Peters T
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49 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.43.2.237
Published
1969-11-00
Pages
237-49
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107870
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.43.2.237 PMC Full Text View journal details
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