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PMID: 5340368 Published · ppublish English Journal Article

The primary structure of porcine pancreatic elastase. The N-terminus and disulphide bridges.

The Biochemical journal ·Vol. 103 ·No. 2 ·1967-05-00 ·Pages 497-507

Brown JR, Kauffman DL, Hartley BS

Abstract

1. The amino acid composition and N-terminal groups of purified elastase show that it is a single peptide chain of 234 residues. 2. The N-terminal sequence is Val-Val-Gly-Gly-Thr-Glu-. 3. The sequences around the four disulphide bridges were determined by using a ;diagonal' electrophoretic technique. 4. These four bridges are homologous with the four common to bovine trypsin and chymotrypsin. 5. Out of 83 residues of the elastase sequence so far determined, 43 are homologous with similar regions of trypsin and chymotrypsin. 6. The evolutionary ancestry of these enzymes is discussed.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Animals Autoanalysis Carboxypeptidases Cattle Chemical Phenomena Chemical Precipitation Chemistry Chromatography Chromatography, Gel Chymotrypsin/analysis Cysteine Cystine/analysis Electrophoresis Endopeptidases Isoflurophate Nitrogen/analysis Pancreatic Elastase/analysis Papain Pepsin A Peptides/analysis Protein Hydrolysates Spectrophotometry Sulfides/analysis Swine Thiocyanates Trichloroacetic Acid Trypsin
Chemicals
Amino Acids Peptides Protein Hydrolysates Sulfides Thiocyanates Isoflurophate Cystine Trichloroacetic Acid Carboxypeptidases Endopeptidases Chymotrypsin Pancreatic Elastase Trypsin Papain Pepsin A Cysteine Nitrogen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brown J R
Kauffman D L
Hartley B S
References (19)
19 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030497
Published
1967-05-00
Pages
497-507
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270434
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030497 PMC Full Text View journal details
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