Influence of culture media on the radiation resistance of Micrococcus radiodurans.

Applied microbiology ·Vol. 15 ·No. 1 ·1967-01-00 ·Pages 178-85

Krabbenhoft KL, Anderson AW, Elliker PR

Abstract

The addition of NZ-case (a tryptic digest of casein) to a growth medium (PC) consisting of tryptone, glucose, and yeast extract caused a significant decrease in gamma radiation resistance of Micrococcus radiodurans. The level of radiation resistance was inversely related to the concentration of NZ-case. The ld(50) for this organism was approximately 700 krad when grown in tryptone, glucose, yeast extract, and dl-methionine (TGYM) broth, but it was approximately one-half as resistant when grown in a PC medium containing 0.5% NZ-case (PCNZ). The resistance to ultraviolet light was also reduced. Cultures transferred from PCNZ to TGYM media regained the high level of resistance.

MeSH Terms

Caseins/pharmacology Culture Media DNA, Bacterial/analysis Micrococcus/analysis,radiation effects Microscopy, Phase-Contrast RNA, Bacterial/analysis Radiation Effects Ultraviolet Rays

Chemicals

Caseins Culture Media DNA, Bacterial RNA, Bacterial

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Krabbenhoft K L

Anderson A W

Elliker P R

References (14)

14 references, click to expand

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The relationship between carotenoid pigments and resistance to radiation in non-photosynthetic bacteria.
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Studies on a radiation-resistant pigmented Sarcina sp.
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Radiat Res. 1964 May;22:155-64 PMID: 14157356
Restoration of Escherichia coli strain B after irradiation: its dependence on suboptimal growth conditions.
J Gen Microbiol. 1958 Apr;18(2):461-72 PMID: 13525662
Similarity of repair of ionizing and ultra-violet radiation damage in Micrococcus radiodurans.
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THE RESISTANCE OF MICROCOCCUS RADIODURANS TO ULTRAVIOLET RADIATION. I. ULTRAVIOLET-INDUCED LESIONS IN THE CELL'S DNA.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.15.1.178-185.1967

Published

1967-01-00

Pages

178-85

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC546865

Subset

External Links

PubMed source DOI: 10.1128/am.15.1.178-185.1967 PMC Full Text

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Influence of culture media on the radiation resistance of Micrococcus radiodurans.

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