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PMID: 533772 Published · ppublish English Journal Article

Synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in Veillonella, Selenomonas, and Bacteriodes species.

Journal of bacteriology ·Vol. 140 ·No. 3 ·1979-12-00 ·Pages 980-6

Allison MJ, Robinson IM, Baetz AL

Abstract

Evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. Cultures were grown in media containing unlabeled energy substrates plus [14C]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. Our results indicate alpha-ketoglutarate synthase functions in Selenomonas ruminantium, Veillonella alcalescens, Bacteroides fragilis, Bacteroides vulgatus, Bacteroides uniformis, Bacteroides distasonis, and Bacteroides multiacidus. Evidence for this carboxylation was not found in strains representative of 10 other species.

MeSH Terms
Amino Acids/biosynthesis Anaerobiosis Bacteroides/metabolism Gram-Negative Anaerobic Bacteria/metabolism Ketoglutaric Acids/biosynthesis Ketone Oxidoreductases/metabolism Species Specificity Succinates/metabolism Veillonella/metabolism
Chemicals
Amino Acids Ketoglutaric Acids Succinates Ketone Oxidoreductases 2-oxoglutarate synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Allison M J
Robinson I M
Baetz A L
References (28)
28 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.140.3.980-986.1979
Published
1979-12-00
Pages
980-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216742
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.140.3.980-986.1979 PMC Full Text View journal details
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