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PMID: 5331371 Published · ppublish English Journal Article

Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis.

Science (New York, N.Y.) ·Vol. 153 ·No. 3740 ·1966-09-02 ·Pages 1119-20

Ron EZ, Kohler RE, Davis BD

Abstract

Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

MeSH Terms
Bile Acids and Salts Escherichia coli Freezing Muramidase Ribosomes Ultracentrifugation
Chemicals
Bile Acids and Salts Muramidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ron E Z
Kohler R E
Davis B D
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.153.3740.1119
Published
1966-09-02
Pages
1119-20
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.153.3740.1119 View journal details
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