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PMID: 5323605 Published · ppublish English Journal Article

Strandedness of Vicia faba chromosomes as revealed by enzyme digestion studies.

The Journal of cell biology ·Vol. 26 ·No. 1 ·1965-07-00 ·Pages 125-35

Trosko JE, Wolff S

Abstract

Chromosomes and nuclei isolated from neutral formalin-fixed Vicia faba lateral roots were treated with trypsin, pepsin, RNase, or DNase. Only trypsin affected the morphology of the chromosomes and nuclei. The appearance of the chromosomes after trypsin digestion indicated that each chromatid contained four strands that could be seen with an ordinary light microscope. The experiments are interpreted as indicating that mitotic chromosomes of Vicia faba are multistranded and that the linear continuity of the chromosome is dependent on protein.

MeSH Terms
Autoradiography Chromosomes DNA/metabolism Deoxyribonucleases In Vitro Techniques Microscopy, Phase-Contrast Pepsin A Plant Cells Ribonucleases Trypsin
Chemicals
DNA Deoxyribonucleases Ribonucleases Trypsin Pepsin A
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Trosko J E
Wolff S
References (19)
19 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
Published
1965-07-00
Pages
125-35
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2106708
Subset
IM
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