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PMID: 5322460 Published · ppublish English Journal Article

Ultrastructural interrelationships of nerve processes and smooth muscle cells in three dimensions.

The Journal of cell biology ·Vol. 28 ·No. 1 ·1966-01-00 ·Pages 37-49

Thaemert JC

Abstract

The muscularis externa of the intestinal wall of frogs was fixed in osmium tetroxide, embedded in Vestopal-W, serially sectioned for electron microscopy, and stained with uranyl acetate. A method to obtain individually mounted and properly positioned serial sections is described. The three-dimensional techniques used during the course of this investigation demonstrate that it is possible to examine carefully relatively large areas of tissue on individual serial sections with the electron microscope and subsequently to construct montages of electron micrographs of pertinent areas from each section. Several carefully rendered interrelationships of nerve processes and smooth muscle cells in three dimensions are exhibited and described. Recent studies of other neuro-effector relationships are discussed in relation to the present status of the nature and organization of the autonomic nervous system in visceral organs.

MeSH Terms
Animals Anura Histological Techniques In Vitro Techniques Microscopy, Electron Muscle, Smooth/cytology Myenteric Plexus/anatomy & histology Neuromuscular Junction/anatomy & histology
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Thaemert J C
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51 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.28.1.37
Published
1966-01-00
Pages
37-49
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2106893
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.28.1.37 PMC Full Text View journal details
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