Fractionation of liver chromatin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 2941-4

Marushige K, Bonner J

Abstract

Rat liver chromatin has been fractionated into two fractions on the basis of precipitability in standard saline after mild treatment with DNase II. The major portion of liver chromatin contains little nonhistone protein and is enriched in histones, while a minor portion of such chromatin, with which RNA polymerase is associated, is highly enriched in proteins other than histone and impoverished in histones. Although the relative concentrations of the several histone species present in two chromatin fractions are identical, binding of histones to DNA in the minor fractions appears to be modified, presumably by the presence of proteins other than histones.

MeSH Terms

Animals Centrifugation, Density Gradient Chromatin/analysis DNA/analysis Deoxyribonucleases Electrophoresis Histones/analysis Hot Temperature Liver/analysis Molecular Weight RNA Nucleotidyltransferases Rats Solubility

Chemicals

Chromatin Histones DNA RNA Nucleotidyltransferases Deoxyribonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Marushige K

Bonner J

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.2941

Published

1971-12-00

Pages

2941-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389565

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.2941 PMC Full Text View journal details

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