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PMID: 5288766 Published · ppublish English Journal Article

Translocation of messenger RNA and "accommodation" of fMet-tRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 8 ·1971-08-00 ·Pages 1791-5

Thach SS, Thach RE

Abstract

Messenger RNA is moved a distance of approximately three nucleotides in the 5' direction relative to the ribosome during the translocation of peptidyl-tRNA from the A to the P site. This movement is catalyzed by G factor and is dependent on the hydrolysis of GTP. In contrast, mRNA is not moved during the f(2)-catalyzed hydrolysis of GTP that is involved in the activation of ribosome-bound fMet-tRNA. This second type of GTP-dependent reaction has been named "Accommodation".

MeSH Terms
Carbon Isotopes Chromatography, Paper Formates Genetic Code Guanosine Triphosphate/metabolism Hydrolysis Methionine Models, Chemical Models, Structural Nucleotides/analysis Peptide Biosynthesis Peptide Chain Elongation, Translational Peptide Chain Initiation, Translational Puromycin/pharmacology RNA, Messenger/metabolism RNA, Transfer/metabolism Ribosomes/drug effects,metabolism Tritium
Chemicals
Carbon Isotopes Formates Nucleotides RNA, Messenger Tritium Puromycin Guanosine Triphosphate RNA, Transfer Methionine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Thach S S
Thach R E
References (32)
32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.68.8.1791
Published
1971-08-00
Pages
1791-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389294
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.68.8.1791 PMC Full Text View journal details
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