Turnover of genetically defined catalase isozymes in maize.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 7 ·1971-07-00 ·Pages 1402-6

Quail PH, Scandalios JG

Abstract

The turnover of two homotetramers of catalase, V(4) and Z(4), specified by genes at two separate loci, has been studied during germination of maize seed by the techniques of density labeling and starch-gel electrophoresis. Both isozymes were shown to be turning over during this time. However, Z(4) accumulates because the rate of synthesis exceeds the rate of degradation, whereas V(4) slowly disappears because the rate of degradation exceeds the rate of synthesis. Evidence is also presented that the rate of synthesis of Z(4) exceeds that of V(4), which suggests that this may be a major factor in the differential expression of the two catalase genes.

MeSH Terms

Catalase/biosynthesis,metabolism Centrifugation, Density Gradient Deuterium Electrophoresis Isoenzymes/biosynthesis,metabolism Molecular Biology Nitrates/metabolism Nitrogen Isotopes Plant Development Plant Proteins/biosynthesis,metabolism Plants/enzymology Potassium/metabolism Time Factors Zea mays/enzymology,growth & development

Chemicals

Isoenzymes Nitrates Nitrogen Isotopes Plant Proteins Deuterium Catalase Potassium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Quail P H

Scandalios J G

References (11)

11 references, click to expand

Peroxidase isozymes from horseradish roots. I. Isolation and physical properties.
J Biol Chem. 1966 May 10;241(9):2166-72 PMID: 5946638
Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall.
Science. 1969 Jul 25;165(3891):358-67 PMID: 17809513
Synthesis and inactivation of aminoacyl-transfer RNA synthetases during growth of Escherichia coli.
J Mol Biol. 1969 Aug 14;43(3):529-50 PMID: 5401233
Control of enzyme levels in animal tissues.
Annu Rev Biochem. 1970;39:929-76 PMID: 4394639
Combined gradient-gel electropooresis procedures for determining buoyant densities or sedimentation coefficients of all multiple forms of an enzyme simultaneously.
Anal Biochem. 1971 Feb;39(2):344-55 PMID: 5555465
A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1520-6 PMID: 5237885
Subunit dissociation and recombination of catalase isozymes.
Proc Natl Acad Sci U S A. 1965 May;53(5):1035-40 PMID: 5222545
Determination of catalase activity by means of the Clark oxygen electrode.
Biochim Biophys Acta. 1967 May 16;139(1):171-3 PMID: 6033467
Separation of labeled from unlabeled proteins by equilibrium density gradient sedimentation.
Anal Biochem. 1962 Dec;4:489-504 PMID: 13955327
Synthesis and turnover of nitrate reductase induced by nitrate in cultured tobacco cells.
J Biol Chem. 1971 Mar 25;246(6):1772-9 PMID: 5547704
Deuterium oxide as a density label of peroxidases in germinating barley embryos.
Plant Physiol. 1970 Feb;45(2):148-52 PMID: 16657293

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.7.1402

Published

1971-07-00

Pages

1402-6

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389204

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.7.1402 PMC Full Text View journal details

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